1887

Abstract

Among strains isolated from Korean agricultural soils, four strains (Ps 9-14 group: Ps 1-2, Ps 1-10, Ps 5-5 and Ps 9-14) from the Suwon, Goesan and Samchok regions, three strains (Ps 3-10 group: Ps 2-22, Ps 3-1 and Ps 3-10) from Umsong Region and four strains (Pss 26 group: Pss 14, Pss 25, Pss 26 and Pss 27) from Jinju Region were identified as three independent groups on the basis of 16S rDNA sequence analysis. While, on the basis of 16S rDNA sequence analysis, Ps 9-14 and Ps 3-10 form a phyletic line with CIP 105274, ‘’ IAM 1155 and DSM 11363, Pss 26 is grouped with ATCC 13674 and IAM 1439. According to DNA–DNA hybridization studies, strain Ps 9-14 shows high DNA relatedness to strain Ps 3-10 (52 %) and CIP 105470 (49 %) and strain Ps 3-10 reveals high relatedness to strain Ps 9-14 (48 %) and CIP 105274 (45 %). Strain Pss 26 shows high relatedness to LMG 18378 (54 %), ATCC 33634 (48 %) and LMG 1242 (48 %). On the basis of phenotypic and genotypic analyses, three novel species of the genus are proposed: sp. nov. (type strain Ps 9-14 =LMG 21318 =KACC 10848) for the Ps 9-14 group, sp. nov. (type strain Ps 3-10 =LMG 21317 =KACC 10847) for the Ps 3-10 group and sp. nov. (type strain Pss 26 =LMG 21316 =KACC 10760) for the Pss 26 group.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02326-0
2003-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/1/ijs530021.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02326-0&mimeType=html&fmt=ahah

References

  1. Achouak W., Sutra L., Heulin T., Meyer J.-M., Fromin N., Degraeve S., Christen R., Gardan L. 2000; Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov. two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana . Int J Syst Evol Microbiol 50:9–18 [CrossRef]
    [Google Scholar]
  2. Andersen S. M., Johnsen K., Sørensen J., Nielsen P., Jacobsen C. S. 2000; Pseudomonas frederiksbergensis sp. nov., isolated from soil at a coal gasification site. Int J Syst Evol Microbiol 50:1957–1964 [CrossRef]
    [Google Scholar]
  3. Anzai Y., Kudo Y., Oyaizu H. 1997; The phylogeny of the genera Chryseomonas , Flavimonas , and Pseudomonas supports synonymy of these three genera. Int J Syst Bacteriol 47:249–251 [CrossRef]
    [Google Scholar]
  4. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  5. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.editors 1987 Current Protocols in Molecular Biology New York: Greene/Wiley Interscience;
    [Google Scholar]
  6. Behrendt U., Ulrich A., Schumann P., Erler W., Burghardt J., Seyfarth W. 1999; A taxonomic study of bacteria isolated from grasses: a proposed new species Pseudomonas graminis sp. nov. Int J Syst Bacteriol 49:297–308 [CrossRef]
    [Google Scholar]
  7. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  8. Champion A. B., Barrett E. L., Palleroni N. J., Soderberg K. L., Kunisawa R., Contopoulou R., Wilson A. C., Doudoroff M. 1980; Evolution in Pseudomonas fluorescens . J Gen Microbiol 120:485–511
    [Google Scholar]
  9. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr Microbiol 4:325–330 [CrossRef]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Kato K., Itoh K. 1983; New selective media for Pseudomonas strains producing fluorescent pigment. Soil Sci Plant Nutr 29:525–532 [CrossRef]
    [Google Scholar]
  12. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44:301–307
    [Google Scholar]
  13. Kumar S., Tamura K., Nei M. 1993 mega: molecular evolutionary genetics analysis, version 1.0 University Park, PA: Pennsylvania State University;
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  15. Migula W. 1894; Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1:235–238
    [Google Scholar]
  16. Moore E. R. B., Mau M., Arnscheidt A., Böttger E. C., Hutson R. A., Collins M. D., Van de Peer Y., De Wachter R., Timmis K. N. 1996; The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu stricto) and estimation of the natural intrageneric relationships. Syst Appl Microbiol 19:478–492 [CrossRef]
    [Google Scholar]
  17. Palleroni N. J. 1984; Genus I. Pseudomonas Migula 1894, 237AL. In Bergey's Manual of Systematic Bacteriology vol. 1 pp 141–199Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  18. Palleroni N. J. 1993; Pseudomonas classification. A new case history in the taxonomy of gram-negative bacteria. Antonie van Leeuwenhoek 64:231–251
    [Google Scholar]
  19. Palleroni N. J., Ballard R. W., Ralston E., Doudoroff M. 1972; Deoxyribonucleic acid homologies among some Pseudomonas species. J Bacteriol 110:1–11
    [Google Scholar]
  20. Palleroni N. J., Kunisawa R., Contopoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas . Int J Syst Bacteriol 23:333–339 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Sands D. C., Schroth M. N., Hildebrand D. C. 1970; Taxonomy of phytopathogenic pseudomonads. J Bacteriol 101:9–23
    [Google Scholar]
  23. Schaad N. W. 1988 Laboratory Guide for Identification of Plant Pathogenic Bacteria , 2nd edn. St Paul, MN: APS Press;
    [Google Scholar]
  24. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa , Bacillus macerans , Bacillus azotofixans , and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [CrossRef]
    [Google Scholar]
  25. Seubert W. 1960; Degradation of isoprenoid compounds by microorganisms. I. Isolation and characterization of an isoprenoid-degrading bacterium, Pseudomonas citronellolis n. sp. J Bacteriol 79:426–434
    [Google Scholar]
  26. Sikorski J., Stackebrandt E., Wackernagel W. 2001; Pseudomonas kilonensis sp. nov., a bacterium isolated from agricultural soil. Int J Syst Evol Microbiol 51:1549–1555
    [Google Scholar]
  27. Sneath P. H. A., Stevens M., Sackin M. J. 1981; Numerical taxonomy of Pseudomonas based on published records of substrate utilization. Antonie van Leeuwenhoek 47:423–448 [CrossRef]
    [Google Scholar]
  28. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  29. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43:159–271 [CrossRef]
    [Google Scholar]
  30. Verhille S., Baida N., Dabboussi F., Izard D., Leclerc H. 1999; Taxonomic study of bacteria isolated from natural mineral waters: proposal of Pseudomonas jessenii sp. nov. and Pseudomonas mandelii sp. nov. Syst Appl Microbiol 22:45–58 [CrossRef]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R.9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  32. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02326-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02326-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error