1887

Abstract

Phylogenetic relationships of five jujube witches'-broom (JWB) phytoplasma isolates from four different districts, and other phytoplasmas, were investigated by 16S rDNA PCR amplification and sequence analysis. The 16S rDNA sequences of any pair of the five isolates of JWB phytoplasmas were >99·5 % similar. The JWB phytoplasma 16S rDNA sequences were most closely related to that of the elm yellows (EY) phytoplasma in 16S-group VIII. Phylogenetic analysis of the 16S rDNA sequences from the JWB phytoplasma isolates, together with sequences from most of the phytoplasmas archived in GenBank, produced a tree in which the JWB isolates clustered as a discrete subgroup. The uniqueness of the JWB phytoplasma appears to be correlated with a specific insect vector ( ) and the host plant ( ), or with a specific geographical distribution. The unique properties of the JWB phytoplasma sequences clearly indicate that it represents a novel taxon, ‘ Phytoplasma ziziphi’.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02393-0
2003-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/4/ijs531037.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02393-0&mimeType=html&fmt=ahah

References

  1. Gundersen D. E., Lee I.-M., Rehner S. A., Davis R. E., Kingsbury D. T. 1994; Phylogeny of mycoplasmalike organisms (phytoplasmas): a basis for their classification. J Bacteriol 176:5244–5254
    [Google Scholar]
  2. Jung H.-Y., Sawayanagi T., Kakizawa S.7 other authors 2002; Candidatus Phytoplasma castaneae’, a novel phytoplasma taxon associated with chestnut witches' broom disease. Int J Syst Evol Microbiol 52:1543–1549 [CrossRef]
    [Google Scholar]
  3. Kim C.-J. 1965; Witches' broom of jujube tree ( Zizyphus jujuba Mill. var. inermis Rehd.). Transmission by grafting. Korean J Microbiol 3:1–6
    [Google Scholar]
  4. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  5. La Y.-J., Woo K.-S. 1980; Transmission of jujube witches' broom mycoplasma by the leaf hopper Hishimonus sellatus Uhler. J Korean For Soc 48:29–39
    [Google Scholar]
  6. Lee J.-T. 1988; Investigation on jujube diseases and their severities of incidence. Res Rept RDA (Agri Institutional Cooperation 31155–161
  7. Lee I.-M., Gundersen D. E., Davis R. E., Chiykowski L. N. 1992; Identification and analysis of a genomic strain cluster of mycoplasmalike organisms associated with Canadian peach (eastern) X disease, western X disease, and clover yellow edge. J Bacteriol 174:6694–6698
    [Google Scholar]
  8. Lee I.-M., Gundersen D. E., Hammond R. W., Davis R. E. 1994; Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology 84:559–566 [CrossRef]
    [Google Scholar]
  9. Lee I.-M., Gundersen-Rindal D. E., Bertaccini A. 1998; Phytoplasma: ecology and genomic diversity. Phytopathology 88:1359–1366 [CrossRef]
    [Google Scholar]
  10. Namba S., Kim Y.-H., Tsuchizaki T. 1993a; Phylogenetic comparison of MLOs occurring in Korea and Japan. Ann Phytopathol Soc Jpn 59:726
    [Google Scholar]
  11. Namba S., Kato S., Iwanami S., Oyaizu H., Shiozawa H., Tsuchizaki T. 1993b; Detection and differentiation of plant-pathogenic mycoplasma-like organisms using polymerase chain reaction. Phytopathology 83:786–791 [CrossRef]
    [Google Scholar]
  12. Namba S., Oyaizu H., Kato S., Iwanami S., Tsuchizaki T. 1993c; Phylogenetic diversity of phytopathogenic mycoplasmalike organisms. Int J Syst Bacteriol 43:461–467 [CrossRef]
    [Google Scholar]
  13. Ohashi A., Nohira T., Yamaguchi K., Kusunoki M., Shiomi T. 1996; Jujube ( Zizyphus jujuba) witches' broom caused by phytoplasma in Gifu prefecture. Trans For Soc Jpn 107:309–310
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Sawayanagi T., Horikoshi N., Kanehira T., Shinohara M., Bertaccini A., Cousin M.-T., Hiruki C., Namba S. 1999; Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanase Hydrangea phyllody. Int J Syst Bacteriol 49:1275–1285 [CrossRef]
    [Google Scholar]
  16. Seemüller E., Marcone C., Lauer U., Ragozzino A., Göschl M. 1998; Current status of molecular classification of the phytoplasmas. J Plant Pathol 80:3–26
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Tian J. B., Guo H. P., Bertaccini A., Martini M., Paltrinieri S., Pastore M. 2000; Molecular detection of jujube witches' broom phytoplasmas in micropropagated jujube shoots. Hortic Sci 35:1274–1275
    [Google Scholar]
  19. Yi C.-K., La Y.-J. 1973; Mycoplasma-like bodies found in the phloem elements of jujube infected with witches' broom disease. J Korean For Soc 20:111–114
    [Google Scholar]
  20. Zhu S. F., Lee I.-M., Gundersen D. E., Zhang C. L., Hadidi A. E. 1996; Phytoplasmas associated with cherry lethal yellows and jujube witches' broom in China represent a new Candidatus subspecies level taxon. IOM Lett 4:218
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02393-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02393-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error