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Volume 53, Issue 2

sp. nov., a novel marine bacterium that produces phenolic anti-methicillin-resistant substances Free

Abstract

Four strains of aerobic, Gram-negative rods, motile by means of a single polar flagellum, that produced phenolic anti-methicillin-resistant (MRSA) substances and brown-pigmented colonies, were isolated from sea water. The G+C content of the DNA ranged from 39·9 to 40·6 mol%. The isolates grew at 18–37 °C and pH 6·5–9·5 (optimal pH 7·5–9) and in medium containing 1–5 % (w/v) NaCl (optimal NaCl concentration 2–3·5 %). The isolates grew optimally in medium dissolved in 40–100 % artificial sea water. Based on 16S rDNA similarities, the novel strains were closely related to and , with 96·3 and 95·7 % sequence similarity, respectively. However, the strains could be differentiated from by seven traits and from by 10 traits. Analysis of DNA–DNA relatedness to these related species revealed low levels of DNA hybridization (19·6 % to and 22·4 % to ). However, the type strain, O-BC30, and the other three bacterial isolates showed high DNA relatedness to each other, ranging from 84·8 to 93·7 %. Based on the results of phenotypic characterization, phylogenetic analysis based on 16S rDNA sequences and DNA–DNA hybridization, it is concluded that these isolates represent a novel species in the genus . Because the type strain, O-BC30 (=IAM 14989 =KCTC 12086), produces phenolic anti-MRSA substances, the name proposed for this novel species is sp. nov.

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Keyword(s): ASW, artificial sea water and MRSA, methicillin-resistant Staphylococcus aureus
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2003-03-01
2024-04-19
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Pseudoalteromonas phenolica sp. nov., a novel marine bacterium that produces phenolic anti-methicillin-resistant Staphylococcus aureus substances
Int J Syst Evol Microbiol 53, 583 (2003); https://doi.org/10.1099/ijs.0.02431-0
/content/journal/ijsem/10.1099/ijs.0.02431-0
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