1887

Abstract

Two bacterial strains associated with the white-rot fungus were subjected to taxonomic investigation. The isolates, designated KCTC 12081 and KCTC 12082, were Gram-negative, non-motile, non-spore-forming and ovoid to rod-shaped. The strains contained major amounts of hexadecanoic acid, -heptadecanoic acid and -7--octadecenoic acid in their cell envelopes. Strain KCTC 12081 contained ubiquinone-8 as the major isoprenoid quinone and the G+C content of its genomic DNA was 61·3 mol%. Morphological and chemotaxonomic properties of the strains were consistent with classification in the genus . In a comparison of 16S rDNA sequence, KCTC 12081 shared 100 % similarity with KCTC 12082 and both strains formed a distinct phylogenetic lineage within the genus . The two strains were also differentiated from other species of this genus by fatty acid composition and phenotypic properties. DNA–DNA relatedness data further supported the separation of the new isolates from closely related species. It is therefore proposed that strains KCTC 12081 (=JCM 11778) and KCTC 12082 be recognized as a novel species, for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02456-0
2003-09-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/5/ijs531631.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02456-0&mimeType=html&fmt=ahah

References

  1. Coenye T., Laevens S., Willems A., Ohlén M., Hannant W., Govan J. R. W., Gillis M., Falsen E., Vandamme P. 2001a; Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. Int J Syst Evol Microbiol 51:1099–1107 [CrossRef]
    [Google Scholar]
  2. Coenye T., Mahenthiralingam E., Henry D., LiPuma J. J., Laevens S., Gillis M., Speert D. P., Vandamme P. 2001b; Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol 51:1481–1490
    [Google Scholar]
  3. Eriksson J., Hjortstam K., Ryvarden L. 1978 The Corticiaceae of North Europe vol 5 Oslo, Norway: Fungiflora;
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  5. Friedrich M., Grosser R. J., Kern E. A., Inskeep W. P., Ward D. M. 2000; Effect of model sorptive phases on phenanthrene biodegradation: molecular analysis of enrichments and isolates suggests selection based on bioavailability. Appl Environ Microbiol 66:2703–2710 [CrossRef]
    [Google Scholar]
  6. Garzillo A. M., Colao M. C., Caruso C., Caporale C., Celletti D., Buonocore V. 1998; Laccase from the white-rot fungus Trametes trogii . Appl Microbiol Biotechnol 49:545–551 [CrossRef]
    [Google Scholar]
  7. Gillis M., Van Van T., Bardin R.7 other authors 1995; Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int J Syst Bacteriol 45:274–289 [CrossRef]
    [Google Scholar]
  8. Kondo R., Kurashiki K., Sakai K. 1994; In vitro bleaching of hardwood kraft pulp by extracellular enzymes excreted from white rot fungi in a cultivation system using a membrane filter. Appl Environ Microbiol 60:921–926
    [Google Scholar]
  9. Lamar R. T., Larsen M. J., Kirk T. K. 1990; Sensitivity to and degradation of pentachlorophenol by Phanerochaete spp. Appl Environ Microbiol 56:3519–3526
    [Google Scholar]
  10. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  11. Lim Y. W., Kim Y. H., Jung H. S. 2000; The Aphyllophorales of Mungyong Saejae. Mycobiology 28:142–148
    [Google Scholar]
  12. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  13. Nogales B., Moore E. R. B., Llobet-Brossa E., Rossello-Mora R., Amann R., Timmis K. N. 2001; Combined use of 16S ribosomal DNA and 16S rRNA to study the bacterial community of polychlorinated biphenyl-polluted soil. Appl Environ Microbiol 67:1874–1884 [CrossRef]
    [Google Scholar]
  14. Novotný Č., Erbanová P., Cajthaml T., Rothschild N., Dosoretz C., Šašek V. 2000; Irpex lacteus , a white rot fungus applicable to water and soil bioremediation. Appl Microbiol Biotechnol 54:850–853 [CrossRef]
    [Google Scholar]
  15. Palleroni N. J. 1984; Genus I. Pseudomonas Migula 1894, 237AL. In Bergey's Manual of Systematic Bacteriology vol. 1 pp 141–199Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Parke J. L., Gurian-Sherman D. 2001; Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains. Annu Rev Phytopathol 39:225–58 [CrossRef]
    [Google Scholar]
  17. Seigle-Murandi F., Guiraud P., Croize J., Falsen E., Eriksson K. L. 1996; Bacteria are omnipresent on Phanerochaete chrysosporium Burdsall. Appl Environ Microbiol 62:2477–2481
    [Google Scholar]
  18. Vandamme P., Holmes B., Vancanneyt M.8 other authors 1997; Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol 47:1188–1200 [CrossRef]
    [Google Scholar]
  19. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. 1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275 [CrossRef]
    [Google Scholar]
  20. Yamada Y. 1998; Identification of coenzyme Q (ubiquinone) homologs. In The Yeasts – a Taxonomic Study, 4th edn. pp 101–102Edited by Kurtzman J. W., Fell C. P. Amsterdam: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02456-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02456-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error