1887

Abstract

A novel hyperthermophilic, anaerobic archaeon, strain Bio-pl-0405IT2, was isolated from a hydrothermal chimney sample collected from the East Pacific Rise at 2700 m depth in the ‘Sarah Spring’ area (7° 25′ 24″ S 107° 47′ 66″ W). Cells were irregular, motile cocci (0.8–1.5 µm in diameter) and divided by constriction. Growth was observed at temperatures between 60 °C and 95 °C with an optimum at 80 °C. The pH range for growth was between pH 4.0 and pH 8.0 with an optimum around pH 7.0. Strain Bio-pl-0405IT2 grew at salt concentrations of 1–5 % (w/v) NaCl with an optimum at 2 %. The novel isolate grew by fermentation or sulphur respiration on a variety of organic compounds. It was a chemoorganoheterotrophic archaeon growing preferentially with yeast extract, peptone and tryptone as carbon and energy sources and sulphur and organic compounds as electron acceptors; it also grew on maltose and starch. Sulphur or -cystine were required for growth and were reduced to hydrogen sulfide. The strain was resistant to rifampicin, chloramphenicol, vancomycin and kanamycin (all at 100 µg ml) but was sensitive to tetracycline. The G+C content of its genomic DNA was 53.6 mol%. Phylogenetic analysis of the almost complete 16S rRNA gene sequence (1450 bp) of strain Bio-pl-0405IT2 showed that the novel isolate belonged to the genus DNA–DNA hybridization values with the two closest relatives AL662 and JCM 8558 were below the threshold value of 70 %. On the basis of the physiological and genotypic distinctness, we propose a novel species, sp. nov. The type strain is Bio-pl-0405IT2 ( = CSUR P577 = JCM 16307).

Funding
This study was supported by the:
  • PID Origines des Planètes et de la Vie’ CNRS program
  • MENRT
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2013-08-01
2024-03-29
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