1887

Abstract

Sixty-two strains of thermophilic aerobic endospore-forming bacteria were subjected to polyphasic taxonomic study including 16S rRNA gene sequence analysis, polar lipid and fatty acid analysis, phenotypic characterization, and DNA–DNA hybridization experiments. Distinct clusters of the species , , and were formed, allowing their descriptions to be emended, and the distinctiveness of the poorly represented species , and was confirmed. It is proposed that the name be corrected to nom. corrig. clustered between species on the basis of 16S rRNA gene sequence analysis, and its transfer to the genus as comb. nov. (type strain LMG 23032 = DSM 9572 = strain M1 = R-35644) is proposed. The above-mentioned species, together with and , form a monophyletic cluster representing the genus . The distinctiveness of ‘’ was confirmed and it is proposed that it be accommodated, along with , in the genus as sp. nov. (type strain DSM 15730 = ATCC BAA-818 = LMG 26209 = R-35652) and comb. nov. (type strain LMG 26208 = ATCC BAA-942 = DSM 16325 = R-35643), respectively. The type strain of was not closely related to any members of the genera and , and it is proposed that this species be placed in the new genus as gen. nov. comb. nov. The type strain of the type species, , is LMG 23386 ( = DSM 16016 = NCIMB 13995 = Tf = R-35653).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030346-0
2012-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1470.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030346-0&mimeType=html&fmt=ahah

References

  1. Ahmad S., Scopes R. K., Rees G. N., Patel B. K. C. 2000; Saccharococcus caldoxylosilyticus sp. nov., an obligately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 50:517–523 [View Article][PubMed]
    [Google Scholar]
  2. Allan R. N., Lebbe L., Heyrman J., De Vos P., Buchanan C. J., Logan N. A. 2005; Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two new thermoacidophiles isolated from geothermal soils of Northern Victoria Land, Antarctica. Int J Syst Evol Microbiol 55:1039–1050 [View Article][PubMed]
    [Google Scholar]
  3. Ambroz A. 1913; Denitrobacterium thermophilum spec. nova, ein Beitrag zur Biologie der thermophilen Bakterien. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt II 37:3–16
    [Google Scholar]
  4. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small subunit ribosomal RNA sequences. Lett Appl Microbiol 13:202–206 [View Article]
    [Google Scholar]
  5. Banat I. M., Marchant R., Rahman T. J. 2004; Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.. Int J Syst Evol Microbiol 54:2197–2201 [View Article][PubMed]
    [Google Scholar]
  6. Caccamo D., Gugliandolo C., Stackebrandt E., Maugeri T. L. 2000; Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermal vent. Int J Syst Evol Microbiol 50:2009–2012 [View Article][PubMed]
    [Google Scholar]
  7. Chen X. G., Stabnikova O., Tay J. H., Wang J. Y., Tay S. T. 2004; Thermoactive extracellular proteases of Geobacillus caldoproteolyticus, sp. nov., from sewage sludge. Extremophiles 8:489–498 [View Article][PubMed]
    [Google Scholar]
  8. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872. In Bergey’s Manual of Systematic Bacteriology vol. 2 pp. 1105–1140 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  9. Coorevits A., Logan N. A., Dinsdale A. E., Halket G., Scheldeman P., Heyndrickx M., Schumann P., Van Landschoot A., De Vos P. 2011; Bacillus thermolactis sp. nov., isolated from dairy farms, and emended description of Bacillus thermoamylovorans . Int J Syst Evol Microbiol 61:1954–1961 [View Article][PubMed]
    [Google Scholar]
  10. De Clerck E., Rodríguez-Díaz M., Vanhoutte T., Heyrman J., Logan N. A., De Vos P. 2004; Anoxybacillus contaminans sp. nov., and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches. Int J Syst Evol Microbiol 54:941–946 [View Article][PubMed]
    [Google Scholar]
  11. Dinsdale A. E., Halket G., Coorevits A., Van Landschoot A., Busse H. J., De Vos P., Logan N. A. 2011; Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus . Int J Syst Evol Microbiol 61:1802–1810 [View Article][PubMed]
    [Google Scholar]
  12. Donk P. J. 1920; A highly resistant thermophilic organism. J Bacteriol 5:373–374[PubMed]
    [Google Scholar]
  13. Forsyth G., Logan N. A. 2000; Isolation of Bacillus thuringiensis from Northern Victoria Land, Antarctica. Lett Appl Microbiol 30:263–266 [View Article][PubMed]
    [Google Scholar]
  14. Fortina M. G., Mora D., Schumann P., Parini C., Manachini P. L., Stackebrandt E. 2001; Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov.. Int J Syst Evol Microbiol 51:2063–2071 [View Article][PubMed]
    [Google Scholar]
  15. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973 The Genus Bacillus. US Department of Agriculture Handbook no. 427 Washington, DC: Agricultural Research Service;
    [Google Scholar]
  16. Gower J. C. 1971; A general coefficient of similarity and some of its properties. Biometrics 27:857–874 [View Article]
    [Google Scholar]
  17. Gul-Guven R., Guven K., Poli A., Nicolaus B. 2008; Anoxybacillus kamchatkensis subsp. assacharedens subsp. nov., a thermophilic bacterium isolated from a hot spring in Batman. J Gen Appl Microbiol 54:327–334 [View Article][PubMed]
    [Google Scholar]
  18. Kevbrin V. V., Zengler K., Lysenko A. M., Wiegel J. 2005; Anoxybacillus kamchatkensis sp. nov., a novel thermophilic facultative aerobic bacterium with a broad pH optimum from the Geyser valley, Kamchatka. Extremophiles 9:391–398 [View Article][PubMed]
    [Google Scholar]
  19. Klaushofer H., Hollaus F. 1970; Zur Taxonomie der hoch-thermophilen, in Zukerfabriksäften vorkommenden aeroben Sporenbildner. Z Zuckerrind 20:465–470
    [Google Scholar]
  20. Kuisiene N., Raugalas J., Chitavichius D. 2004; Geobacillus lituanicus sp. nov.. Int J Syst Evol Microbiol 54:1991–1995 [View Article][PubMed]
    [Google Scholar]
  21. Kuisiene N., Raugalas J., Chitavichius D. 2009; Phylogenetic, inter, and intraspecific sequence analysis of spo0A gene of the genus Geobacillus . Curr Microbiol 58:547–553 [View Article][PubMed]
    [Google Scholar]
  22. Logan N. A., Berkeley R. C. W. 1984; Identification of Bacillus strains using the API system. J Gen Microbiol 130:1871–1882[PubMed]
    [Google Scholar]
  23. Logan N. A., De Vos P. 2009; Genus Bacillus Cohn 1872. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 3 pp. 21–128 Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. New York: Springer-Verlag;
    [Google Scholar]
  24. Logan N. A., De Vos P., Dinsdale A. 2009; Genus Geobacillus Nazina et al. 2001. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 3 pp. 144–160 Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. New York: Springer-Verlag;
    [Google Scholar]
  25. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar A., Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  26. Manachini P. L., Mora D., Nicastro G., Parini C., Stackebrandt E., Pukall R., Fortina M. G. 2000; Bacillus thermodenitrificans sp. nov., nom. rev.. Int J Syst Evol Microbiol 50:1331–1337 [View Article][PubMed]
    [Google Scholar]
  27. Meintanis C., Chalkou K. I., Kormas K. A., Lymperopoulou D. S., Katsifas E. A., Hatzinikolaou D. G., Karagouni A. D. 2008; Application of rpoB sequence similarity analysis, REP-PCR and BOX-PCR for the differentiation of species within the genus Geobacillus . Lett Appl Microbiol 46:395–401 [View Article][PubMed]
    [Google Scholar]
  28. Miñana-Galbis D., Pinzón D. L., Lorén J. G., Manresa Á., Oliart-Ros R. M. 2010; Reclassification of Geobacillus pallidus (Scholz et al. 1988) Banat et al. 2004 as Aeribacillus pallidus gen. nov., comb. nov.. Int J Syst Evol Microbiol 60:1600–1604 [View Article][PubMed]
    [Google Scholar]
  29. Mishustin E. N. 1950 Termofilnie mikroorganiszmi w prirode I praktike Moscow: Akademii Nauk SSSR;
    [Google Scholar]
  30. Nazina T. N., Tourova T. P., Poltaraus A. B., Novikova E. V., Grigoryan A. A., Ivanova A. E., Lysenko A. M., Petrunyaka V. V., Osipov G. A. other authors 2001; Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans . Int J Syst Evol Microbiol 51:433–446[PubMed]
    [Google Scholar]
  31. Nazina T. N., Lebedeva E. V., Poltaraus A. B., Tourova T. P., Grigoryan A. A., Sokolova D. Sh., Lysenko A. M., Osipov G. A. 2004; Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.. Int J Syst Evol Microbiol 54:2019–2024 [View Article][PubMed]
    [Google Scholar]
  32. Nazina T. N., Sokolova D. Sh., Grigoryan A. A., Shestakova N. M., Mikhailova E. M., Poltaraus A. B., Tourova T. P., Lysenko A. M., Osipov G. A., Belyaev S. S. 2005; Geobacillus jurassicus sp. nov., a new thermophilic bacterium isolated from a high-temperature petroleum reservoir, and the validation of the Geobacillus species. Syst Appl Microbiol 28:43–53 [View Article][PubMed]
    [Google Scholar]
  33. Nicolaus B., Lama L., Esposito E., Manca M. C., di Prisco G., Gambacorta A. 1996; Bacillus thermoantarcticus” sp. nov., from Mount Melbourne, Antarctica: a novel thermophilic species. Polar Biol 16:101–104
    [Google Scholar]
  34. Pikuta E. V. 2009; Genus Anoxybacillus Pikuta et al. 2000. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 3 pp. 134–141 Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B. New York: Springer-Verlag;
    [Google Scholar]
  35. Poli A., Romano I., Caliendo G., Nicolaus G., Orlando P., Falco A., Lama L., Gambacorta A., Nicolaus B. 2006; Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost. J Gen Appl Microbiol 52:223–234 [View Article][PubMed]
    [Google Scholar]
  36. Poli A., Romano I., Cordella P., Orlando P., Nicolaus B., Ceschi Berrini C. 2009; Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy. Extremophiles 13:867–874 [View Article][PubMed]
    [Google Scholar]
  37. Schäffer C., Franck W. L., Scheberl A., Kosma P., McDermott T. R., Messner P. 2004; Classification of isolates from locations in Austria and Yellowstone National Park as Geobacillus tepidamans sp. nov.. Int J Syst Evol Microbiol 54:2361–2368 [View Article][PubMed]
    [Google Scholar]
  38. Scholz T., Demharter W., Hensel R., Kandler O. 1987; Bacillus pallidus sp. nov., a new thermophilic species from sewage. Syst Appl Microbiol 9:91–96 [View Article]
    [Google Scholar]
  39. Scholz T., Demharter W., Hensel R., Kandler O. 1988; Bacillus pallidus sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 24. Int J Syst Bacteriol 38:136–137 [View Article]
    [Google Scholar]
  40. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int J Syst Bacteriol 30:225–420 [View Article]
    [Google Scholar]
  41. Slabbinck B., Dawyndt P., Martens M., De Vos P., De Baets B. 2008; TaxonGap: a visualization tool for intra- and inter-species variation among individual biomarkers. Bioinformatics 24:866–867 [View Article][PubMed]
    [Google Scholar]
  42. Sung M. H., Kim H., Bae J. W., Rhee S. K., Jeon C. O., Kim K., Kim J. J., Hong S. P., Lee S. G. other authors 2002; Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost. Int J Syst Evol Microbiol 52:2251–2255 [View Article][PubMed]
    [Google Scholar]
  43. Suzuki Y., Kishigami T., Inoue K., Mizoguchi Y., Eto N., Takagi M., Abe S. 1983; Bacillus thermoglucosidasius sp. nov., a new species of obligately thermophilic bacilli. Syst Appl Microbiol 4:487–495 [View Article]
    [Google Scholar]
  44. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  45. Tourova T. P., Korshunova A. V., Mikhailova E. M., Sokolova D. Sh., Poltaraus A. B., Nazina T. N. 2010; Application of gyrB and parE sequence similarity analyses for differentiation of species within the genus Geobacillus . Microbiology (English translation of Mikrobiologiia) 79:356–369 [View Article]
    [Google Scholar]
  46. Vauterin L., Yang P., Hoste B., Vancanneyt M., Civerolo E. L., Swings J., Kersters K. 1991; Differentiation of Xanthomonas campestris pv. citri strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, fatty acid analysis, and DNA-DNA hybridization. Int J Syst Bacteriol 41:535–542 [View Article]
    [Google Scholar]
  47. Walker P. D., Wolf J. 1971; The taxonomy of Bacillus stearothermophilus . In Spore Research 1971 pp. 247–262 Edited by Barker A. N., Gould G. W., Wolf J. London: Academic Press;
    [Google Scholar]
  48. Weng F. Y., Chiou C. S., Lin P. H. P., Yang S. S. 2009; Application of recA and rpoB sequence analysis on phylogeny and molecular identification of Geobacillus species. J Appl Microbiol 107:452–464 [View Article][PubMed]
    [Google Scholar]
  49. White D., Sharp R. J., Priest F. G. 1993; A polyphasic taxonomic study of thermophilic bacilli from a wide geographical area. Antonie van Leeuwenhoek 64:357–386 [View Article][PubMed]
    [Google Scholar]
  50. Zeigler D. R. 2005; Application of a recN sequence similarity analysis to the identification of species within the bacterial genus Geobacillus . Int J Syst Evol Microbiol 55:1171–1179 [View Article][PubMed]
    [Google Scholar]
  51. Zhou Y., Wei W., Che Q., Xu Y., Wang X., Huang X., Lai R. 2008; Bacillus pallidus sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 58:2850–2854 [View Article][PubMed]
    [Google Scholar]
  52. Zhou Y., Xu J., Xu L., Tindall B. J. 2009; Falsibacillus pallidus to replace the homonym Bacillus pallidus Zhou et al. 2008. Int J Syst Evol Microbiol 59:3176–3180 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030346-0
Loading
/content/journal/ijsem/10.1099/ijs.0.030346-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error