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Volume 63, Issue Pt_1

Transfer of Johnson 1956 to gen. nov., in the family , as gen. nov., comb. nov. Free

Abstract

Johnson 1956, a plant-pathogenic bacterium on the pods of the French bean, is no longer considered to be a member of the genus . A polyphasic approach that included examination of phenotypic properties and phylogenetic analyses based on 16S rRNA, and gene sequences supported the transfer of Johnson 1956 to a new genus in the family as gen. nov., comb. nov. Two strains of were studied (ATCC 12775 and LMG 2186); the strains shared 99.8 % sequence similarity in their 16S rRNA genes and the housekeeping gene sequences were identical. Strains of shared 96.6 % or less 16S rRNA gene sequence similarity with members of different genera in the family and only 84.7 % sequence similarity with LMG 1242, demonstrating that they are not related to the genus . As formed an independent phyletic lineage in all of the phylogenetic analyses, it could not be affiliated to any of the recognized genera within the family and therefore was assigned to a new genus. Cells were Gram-negative, straight rods, motile by means of one or two polar flagella, fermentative, facultative anaerobes, oxidase-negative and catalase-positive. Growth occurred in the presence of 0–60 % sucrose. The DNA G+C content of the type strain was 44.3 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, Johnson 1956 is transferred to the novel genus gen. nov. as gen. nov., comb. nov. The type strain of is ATCC 12775  = CFBP 3281  = ICMP 745  = LMG 2187  = NCPPB 539.

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Funding
This study was supported by the:
  • Israel Science Foundation (Award 189/08)
© 2013 IUMS
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2013-01-01
2024-04-19
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Transfer of Pseudomonas flectens Johnson 1956 to Phaseolibacter gen. nov., in the family Enterobacteriaceae, as Phaseolibacter flectens gen. nov., comb. nov.
Int J Syst Evol Microbiol 63, 268 (2013); https://doi.org/10.1099/ijs.0.033654-0
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