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Volume 62, Issue Pt_12

sp. nov., isolated from hexachlorocyclohexane (HCH)-contaminated soil Free

Abstract

A bacterial strain, designated Dd16, was isolated from a hexachlorocyclohexane (HCH) dumpsite at Lucknow, India. Cells of strain Dd16 were Gram-stain-negative, non-motile, rod-shaped and yellow-pigmented. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus in the family , as it showed highest sequence similarity to IFO 15499 (95.36 %), YC7378 (95.30), ‘’ PB323 (95.20 %), NX02 (95.14 %) and CP1D (95.00 %). The major fatty acids were summed feature 3 (Cω7/Cω6) C 2-OH, summed feature 8 (Cω7 and/or Cω6) and C. The polar lipid profile of strain Dd16 also corresponded to those reported for species of the genus (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, and a sphingoglycolipid), again supporting its identification as a member of the genus . The predominant respiratory quinone was ubiquinone Q, and -homospermidine was the major polyamine observed. The total DNA G+C content of strain Dd16 was 65.8 mol%. The results obtained on the basis of phenotypic characteristics and phylogenetic analysis and after biochemical and physiological tests, clearly distinguished strain Dd16 from closely related members of the genus Thus, strain Dd16 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is Dd16 ( = DSM 25434 = CCM 7882).

  • Published Online:
Funding
This study was supported by the:
  • Department of Biotechnology (DBT), National Bureau of Agriculturally Important Micro-organisms (NBAIM, ICAR)
  • DU/DST-PURSE Grant, Government of India
  • Council for Scientific and Industrial Research (CSIR)
  • Department of Biotechnology (DBT)
© 2012 IUMS
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2012-12-01
2024-04-19
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Sphingomonas indica sp. nov., isolated from hexachlorocyclohexane (HCH)-contaminated soil
Int J Syst Evol Microbiol 62, 2997 (2012); https://doi.org/10.1099/ijs.0.033845-0
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