1887

Abstract

Strain H5 was isolated from a sediment sample collected from the coastal area of Qingdao, China. The cells were Gram-stain-positive, non-motile, straight or curved rods. The temperature range for growth was 20–37 °C and the pH for growth ranged from 6.5 to 9.0, with optimum growth occurring in the temperature range 28–30 °C and pH range 7.5–8.0. Growth occurred in the presence of 0–6 % (w/v) NaCl (optimum, 0–2 %). Strain H5 had MK-9, MK-9(H) and MK-9(H) as the major menaquinones and Cω9, C, C, C and Cω9 as major fatty acids. The cell-wall peptidoglycan type was A5α -Lys--Ala--Lys--Glu. The major polar lipids were phosphatidylglycerol (PG), an unknown phospholipid (PL1) and two unknown phosphoglycolipids (PGL1, PGL2). An unknown phospholipid (PL2) and two unknown glycolipids (GL1, GL2) were present in moderate to minor amounts in the polar lipid profile. The genomic DNA G+C content was 61.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain H5 represents a novel lineage in the family . On the basis of phenotypic, physiological and molecular characteristics, it is proposed that the novel isolate should be classified as a novel species in a new genus: gen. nov., sp. nov., with strain H5 ( = DSM 24315 = CICC 10486) as the type strain of the type species.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 40730847)
  • Shandong Provincial Programs for Science and Technology Development (Award 2011GSF12108)
  • China Postdoctoral Science Foundation (Award 2012M511493)
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2013-05-01
2024-03-29
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