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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_5

gen. nov., sp. nov., epibiotic bacteria isolated from the surface of a marine alga Free

Abstract

Two Gram-reaction-negative, rod-shaped, motile bacteria, designated strains U82 and U95, were isolated from the marine alga collected at Sharks Point, Clovelly, a rocky intertidal zone near Sydney, Australia. Both strains were oxidase- and catalase-positive, formed brown- to black-pigmented colonies and required NaCl for growth. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that these strains belong to the clade within the . The 16S rRNA genes of both strains were identical across the sequenced 1326 nt, but showed differences in the intergenic spacer region (ITS) between the 16S and the 23S rRNA genes. At the genomic level the DNA G+C contents of strains U82 and U95 were identical (52.6 mol%) and they had a DNA–DNA hybridization value of 83.7 %, suggesting that these strains belong to the same species. The closest described phylogenetic neighbour to strains U82 and U95 was DSM 15283 with 95.8 % 16S rRNA gene sequence similarity. Other close relatives include further species of the genera and . Strains U82 and U95 were negative for bacteriochlorophyll production, showed antibacterial activity towards other marine bacteria, were resistant to the antibiotics gentamicin and spectinomycin and were unable to hydrolyse starch or gelatin. The major fatty acids (>1 %) were 18 : 1ω7, 16 : 0, 18 : 2, 10 : 0 3-OH, 12 : 0, 20 : 1 2-OH and 18 : 0. The polar lipid pattern indicated the presence of phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids and four unidentified phospholipids. Both strains produced ubiquinone 10 (Q-10) as the sole respiratory lipoquinone. Based on their phenotypic and phylogenetic characteristics, it is suggested that strains U82 and U95 are members of a novel species within a new genus for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is U95 ( = DSM 24752 = LMG 26464).

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Funding
This study was supported by the:
  • Deutsche Forschungsgemeinschaft
  • Australian Research Council
  • Centre for Marine Bio-Innovation
© 2013 IUMS
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2013-05-01
2024-04-16
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Epibacterium ulvae gen. nov., sp. nov., epibiotic bacteria isolated from the surface of a marine alga
Int J Syst Evol Microbiol 63, 1589 (2013); https://doi.org/10.1099/ijs.0.042838-0
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