1887

Abstract

Taxonomic analysis of five yeast strains isolated from flowers in Brunei (Borneo) is described. The strains represent a dimorphic, biofilm-producing, anamorphic budding yeast species for which the name is proposed. alternates between yeast and pseudohyphal modes of growth. The pseudohyphae form biofilms on the surface of liquid media and penetrate into solid substrates. The sequences of the D1/D2 domains of the large subunit rRNA genes, the internal transcribed spacer (ITS) regions and the 18S rRNA genes were identical in the five strains and indicated a close phylogenetic relationship with teleomorph species of the genus . In a phylogenetic analysis of these sequences, the closest relative of the new species was (6 % nucleotide substitutions and indels in the D1/D2 domain). The type strain is 11-485 and has been deposited in the Centralbureau voor Schimmelcultures (Utrecht, the Netherlands) as CBS 12611, the National Collection of Agricultural and Industrial Micro-organisms (Budapest, Hungary) as NCAIM Y.02019 and the Culture Collection of Yeasts (Bratislava, Slovakia) as CCY 29-189-1. Mycobank no. MB800537.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.044974-0
2012-12-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/12/3099.html?itemId=/content/journal/ijsem/10.1099/ijs.0.044974-0&mimeType=html&fmt=ahah

References

  1. Arias C. R., Burns J. K., Friedrich L. M., Goodrich R. M., Parish M. E. 2002; Yeast species associated with orange juice: evaluation of different identification methods. Appl Environ Microbiol 68:1955–1961 [View Article][PubMed]
    [Google Scholar]
  2. Arteau M., Labrie S., Roy D. 2010; Terminal-restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis profiling of fungal communities in Camembert cheese. Int Dairy J 20:545–554 [View Article]
    [Google Scholar]
  3. Barnett J. A., Payne R. W., Yarrow D. 1990 Yeasts Characteristics and Identification Cambridge, UK: Cambridge University Press;
    [Google Scholar]
  4. Basílio A. C., de Araújo P. R., de Morais J. O., da Silva Filho E. A., de Morais M. A. Jr, Simões D. A. 2008; Detection and identification of wild yeast contaminants of the industrial fuel ethanol fermentation process. Curr Microbiol 56:322–326 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 2007 phylip (phylogeny inference package), version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Felsenstein J., Churchill G. A. 1996; A Hidden Markov Model approach to variation among sites in rate of evolution. Mol Biol Evol 13:93–104 [View Article][PubMed]
    [Google Scholar]
  7. Katoh K., Toh H. 2008; Recent developments in the mafft multiple sequence alignment program. Brief Bioinform 9:286–298 [View Article][PubMed]
    [Google Scholar]
  8. Kurtzman C. P. 2011; Phylogeny of the ascomycetous yeasts and the renaming of Pichia anomala to Wickerhamomyces anomalus . Antonie van Leeuwenhoek 99:13–23 [View Article][PubMed]
    [Google Scholar]
  9. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [View Article][PubMed]
    [Google Scholar]
  10. Lachance M.-A., Bowles J. M., Chavarría Díaz M. M., Janzen D. H. 2001a; Candida cleridarum, Candida tilneyi and Candida powellii, three new yeast species isolated from insects associated with flowers. Int J Syst Evol Microbiol 51:1201–1207 [View Article][PubMed]
    [Google Scholar]
  11. Lachance M.-A., Starmer W. T., Rosa C. A., Bowles J. M., Barker J. S. F., Janzen D. H. 2001b; Biogeography of the yeasts of ephemeral flowers and their insects. FEMS Yeast Res 1:1–8[PubMed] [CrossRef]
    [Google Scholar]
  12. Nilsson R. H., Kristiansson E., Ryberg M., Hallenberg N., Larsson K.-H. 2008; Intraspecific ITS variability in the kingdom fungi as expressed in the international sequence databases and its implications for molecular species identification. Evol Bioinform Online 4:193–201[PubMed]
    [Google Scholar]
  13. O’Donell K. 1993; Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Sytematics pp. 225–233 Edited by Reynolds D. R., Taylor J. W. Wallingford, UK: CAB International;
    [Google Scholar]
  14. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358[PubMed]
    [Google Scholar]
  15. Sipiczki M. 2003; Candida zemplinina sp. nov., an osmotolerant and psychrotolerant yeast that ferments sweet botrytized wines. Int J Syst Evol Microbiol 53:2079–2083 [View Article][PubMed]
    [Google Scholar]
  16. Sipiczki M. 2010; Candida stigmatis sp. nov., a new anamorphic yeast species isolated from flowers. FEMS Yeast Res 10:362–365 [View Article][PubMed]
    [Google Scholar]
  17. Sipiczki M. 2011; Dimorphic cycle in Candida citri sp. nov., a novel yeast species isolated from rotting fruit in Borneo. FEMS Yeast Res 11:202–208 [View Article][PubMed]
    [Google Scholar]
  18. Sipiczki M., Kajdacsi E. 2009; Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales. Int J Syst Evol Microbiol 59:914–920 [View Article][PubMed]
    [Google Scholar]
  19. Sipiczki M., Takeo K., Yamaguchi M., Yoshida S., Miklos I. 1998; Environmentally controlled dimorphic cycle in a fission yeast. Microbiology 144:1319–1330 [View Article][PubMed]
    [Google Scholar]
  20. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  21. van der Walt J. P., Yarrow D. 1984; Methods for the isolation, maintenance, classification and identification of yeasts. In The Yeasts, a Taxonomic Study, 3rd edn. pp. 45–104 Edited by Kreger-van Rij N. J. W. Amsterdam: Elsevier;
    [Google Scholar]
  22. White T. J., Burns T., Lee S., Taylor J. 1990; Amplification and sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols. A Guide to Methods and Applications pp. 315–322 Edited by Innis M. A., Gelfand D. H., Snisky J. J., White T. J. San Diego, CA: Acadamic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.044974-0
Loading
/content/journal/ijsem/10.1099/ijs.0.044974-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error