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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_7

sp. nov., a nitrogen-fixing bacterium associated with sugar cane ( L.) Free

Abstract

Five nitrogen-fixing bacterial strains (SP1, NN143, NN144, NN208 and HX148) were isolated from stem, root or rhizosphere soil of sugar cane ( L.) plants. Cells were Gram-negative, motile, rods with peritrichous flagella. DNA G+C content was 55.0±0.5 mol%. Sequence determinations and phylogenetic analysis of 16S rRNA gene and indicated that the strains were affiliated with the genus and most closely related to DSM 16656 and LMG 24251. Fluorimetric determination of thermal denaturation temperatures after DNA–DNA hybridization, enterobacterial repetitive intergenic consensus PCR and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry differentiated the whole-genome, genotype and protein profiles from those of and . The strains’ cell fatty acid composition differentiated them from and by containing a higher level of summed feature 2 (Cω7 and/or Cω6) and a lower level of C cyclo. Their physiological and biochemical profiles differentiated them from by being positive for methyl red test, ornithine decarboxylase and utilization of putrescine, -arabitol, -fucose and methyl α--glucoside and being negative for arginine dihydrolase, and differentiated them from by being positive for aesculin hydrolysis and utilization of putrescine, -arabitol and -rhamnose and being negative for arginine dihydrolase, lysine decarboxylase and utilization of mucate. The five strains therefore represent a novel species, for which the name sp. nov. is proposed, with the type strain SP1 ( = CGMCC 1.12102 = LMG 26783).

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Funding
This study was supported by the:
  • China Postdoctoral Foundation (Award 317000-X91102)
  • National Natural Science Foundation of China (Award 31171504)
  • Ministry of Agriculture (Award 201003066)
  • Ministry of Science and Technology (Award 2006AA10211)
© 2013 IUMS
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2013-07-01
2024-04-24
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Enterobacter sacchari sp. nov., a nitrogen-fixing bacterium associated with sugar cane (Saccharum officinarum L.)
Int J Syst Evol Microbiol 63, 2577 (2013); https://doi.org/10.1099/ijs.0.045500-0
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