1887

Abstract

A Gram-stain-negative, aerobic, rod-like, motile by peritrichous flagella and moderately halophilic bacterium, designated strain B6, was isolated a deep-sea sediment collected from the South Atlantic Ocean. The isolate grew with 0.5–15 % (w/v) NaCl, at 4–37 °C and pH 5.0–8.5 and showed a high tolerance to zinc, manganese, cobalt and copper ions. The major fatty acids were C, C cyclo ω8, C 3-OH and C. The predominant ubiquinone was Q-9. The genomic DNA G+C content was 61.1 mol%. Phylogenetic analysis based on 16S rRNA gene comparisons indicated that strain B6 belonged to the genus , and the closest relative was TRM 0175 (96.1 %). Based upon the phenotypic, chemotaxonomic and genetic data, strain B6 represents a novel species from the genus , for which the name sp. nov. is proposed. The type strain is B6 ( = CGMCC 1.12450 = JCM 18472).

Funding
This study was supported by the:
  • China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-R-03)
  • Public Science and Technology Research Funds Projects of Ocean (Award 201005032-3)
  • National Natural Science Foundation of China (Award 41276173)
  • Scientific Research Fund of the Second Institute of Oceanography, State Oceanic Administration (Award JT0906)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.051656-0
2013-11-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/11/4230.html?itemId=/content/journal/ijsem/10.1099/ijs.0.051656-0&mimeType=html&fmt=ahah

References

  1. Amjres H., Béjar V., Quesada E., Abrini J., Llamas I. ( 2011 ). Halomonas rifensis sp. nov., an exopolysaccharide-producing, halophilic bacterium isolated from a solar saltern. . Int J Syst Evol Microbiol 61, 26002605. [View Article] [PubMed]
    [Google Scholar]
  2. Arahal D. R., Castillo A. M., Ludwig W., Schleifer K.-H., Ventosa A. ( 2002 ). Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina . . Syst Appl Microbiol 25, 207211. [View Article] [PubMed]
    [Google Scholar]
  3. Arahal D. R., Vreeland R. H., Litchfield C. D., Mormile M. R., Tindall B. J., Oren A., Bejár V., Quesada E., Ventosa A. ( 2007 ). Recommended minimal standards for describing new taxa of the family Halomonadaceae . . Int J Syst Evol Microbiol 57, 24362446. [View Article] [PubMed]
    [Google Scholar]
  4. Arenas M., Bañón P. I., Copa-Patiño J. L., Sánchez-Porro C., Ventosa A., Soliveri J. ( 2009 ). Halomonas ilicicola sp. nov., a moderately halophilic bacterium isolated from a saltern. . Int J Syst Evol Microbiol 59, 578582. [View Article] [PubMed]
    [Google Scholar]
  5. Chen Y.-G., Zhang Y.-Q., Huang H.-Y., Klenk H.-P., Tang S.-K., Huang K., Chen Q.-H., Cui X.-L., Li W.-J. ( 2009 ). Halomonas zhanjiangensis sp. nov., a halophilic bacterium isolated from a sea urchin. . Int J Syst Evol Microbiol 59, 28882893. [View Article] [PubMed]
    [Google Scholar]
  6. de la Haba R. R., Márquez M. C., Papke R. T., Ventosa A. ( 2012 ). Multilocus sequence analysis of the family Halomonadaceae . . Int J Syst Evol Microbiol 62, 520538. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: Minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  10. Garrity G. M., Bell J. A., Lilburn T. ( 2005 ). Family IV. Halomonadaceae Franzmann, Wehmeyer and Stackebrandt 1989, 205VP emend. Dobson and Franzmann, 1996, 558. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, p. 300. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  11. González-Domenech C. M., Béjar V., Martínez-Checa F., Quesada E. ( 2008a ). Halomonas nitroreducens sp. nov., a novel nitrate- and nitrite-reducing species. . Int J Syst Evol Microbiol 58, 872876. [View Article] [PubMed]
    [Google Scholar]
  12. González-Domenech C. M., Martínez-Checa F., Quesada E., Béjar V. ( 2008b ). Halomonas cerina sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. . Int J Syst Evol Microbiol 58, 803809. [View Article] [PubMed]
    [Google Scholar]
  13. González-Domenech C. M., Martínez-Checa F., Quesada E., Béjar V. ( 2009 ). Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium. . Int J Syst Evol Microbiol 59, 12901296. [View Article] [PubMed]
    [Google Scholar]
  14. Guan T.-W., Xiao J., Zhao K., Luo X.-X., Zhang X.-P., Zhang L.-L. ( 2010 ). Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 60, 349352. [View Article] [PubMed]
    [Google Scholar]
  15. Guzmán D., Quillaguamán J., Muñoz M., Hatti-Kaul R. ( 2010 ). Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia. . Int J Syst Evol Microbiol 60, 749753. [View Article] [PubMed]
    [Google Scholar]
  16. Jeon C. O., Lim J.-M., Lee J. R., Lee G. S., Park D.-J., Lee J.-C., Oh H.-W., Kim C.-J. ( 2007 ). Halomonas kribbensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. . Int J Syst Evol Microbiol 57, 21942198. [View Article] [PubMed]
    [Google Scholar]
  17. Kim K. K., Jin L., Yang H. C., Lee S.-T. ( 2007 ). Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water. . Int J Syst Evol Microbiol 57, 675681. [View Article] [PubMed]
    [Google Scholar]
  18. Kim K. K., Lee K. C., Oh H.-M., Lee J.-S. ( 2010a ). Halomonas stevensii sp. nov., Halomonas hamiltonii sp. nov. and Halomonas johnsoniae sp. nov., isolated from a renal care centre. . Int J Syst Evol Microbiol 60, 369377. [View Article] [PubMed]
    [Google Scholar]
  19. Kim M.-S., Roh S. W., Bae J.-W. ( 2010b ). Halomonas jeotgali sp. nov., a new moderate halophilic bacterium isolated from a traditional fermented seafood. . J Microbiol 48, 404410. [View Article] [PubMed]
    [Google Scholar]
  20. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  21. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  22. Komagata K., Suzuki K. ( 1987 ). Lipids and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  23. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [View Article]
    [Google Scholar]
  24. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 11831184.[PubMed]
    [Google Scholar]
  25. Li H.-B., Zhang L.-P., Chen S.-F. ( 2008 ). Halomonas korlensis sp. nov., a moderately halophilic, denitrifying bacterium isolated from saline and alkaline soil. . Int J Syst Evol Microbiol 58, 25822588. [View Article] [PubMed]
    [Google Scholar]
  26. Llamas I., Béjar V., Martínez-Checa F., Martínez-Cánovas M. J., Molina I., Quesada E. ( 2011 ). Halomonas stenophila sp. nov., a halophilic bacterium that produces sulphate exopolysaccharides with biological activity. . Int J Syst Evol Microbiol 61, 25082514. [View Article] [PubMed]
    [Google Scholar]
  27. Luque R., Béjar V., Quesada E., Martínez-Checa F., Llamas I. ( 2012 ). Halomonas ramblicola sp. nov., a moderately halophilic bacterium from Rambla Salada, a Mediterranean hypersaline rambla. . Int J Syst Evol Microbiol 62, 29032909. [View Article] [PubMed]
    [Google Scholar]
  28. Marmur J. ( 1961 ). A procedure of the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  29. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. ( 2002 ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25, 360375. [View Article] [PubMed]
    [Google Scholar]
  30. Menes R. J., Viera C. E., Farías M. E., Seufferheld M. J. ( 2011 ). Halomonas vilamensis sp. nov., isolated from high-altitude Andean lakes. . Int J Syst Evol Microbiol 61, 12111217. [View Article] [PubMed]
    [Google Scholar]
  31. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297306. [View Article] [PubMed]
    [Google Scholar]
  32. Nieto J. J., Fernández-Castillo R., Márquez M. C., Ventosa A., Quesada E., Ruiz-Berraquero F. ( 1989 ). Survey of metal tolerance in moderately halophilic eubacteria. . Appl Environ Microbiol 55, 23852390.[PubMed]
    [Google Scholar]
  33. Poli A., Esposito E., Orlando P., Lama L., Giordano A., de Appolonia F., Nicolaus B., Gambacorta A. ( 2007 ). Halomonas alkaliantarctica sp. nov., isolated from saline lake Cape Russell in Antarctica, an alkalophilic moderately halophilic, exopolysaccharide-producing bacterium. . Syst Appl Microbiol 30, 3138. [View Article] [PubMed]
    [Google Scholar]
  34. Poli A., Nicolaus B., Denizci A. A., Yavuzturk B., Kazan D. ( 2013 ). Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium. . Int J Syst Evol Microbiol 63, 1018. [View Article] [PubMed]
    [Google Scholar]
  35. Qu L., Lai Q., Zhu F., Hong X., Zhang J., Shao Z., Sun X. ( 2011 ). Halomonas daqiaonensis sp. nov., a moderately halophilic, denitrifying bacterium isolated from a littoral saltern. . Int J Syst Evol Microbiol 61, 16121616. [View Article] [PubMed]
    [Google Scholar]
  36. Romano I., Lama L., Nicolaus B., Poli A., Gambacorta A., Giordano A. ( 2006 ). Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy). . J Gen Appl Microbiol 52, 339348. [View Article] [PubMed]
    [Google Scholar]
  37. Romano I., Lama L., Orlando P., Nicolaus B., Giordano A., Gambacorta A. ( 2007 ). Halomonas sinaiensis sp. nov., a novel halophilic bacterium isolated from a salt lake inside Ras Muhammad Park, Egypt. . Extremophiles 11, 789796. [View Article] [PubMed]
    [Google Scholar]
  38. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  39. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  40. Stanier R. Y., Palleroni N. J., Doudoroff M. ( 1966 ). The aerobic pseudomonads: a taxonomic study. . J Gen Microbiol 43, 159271. [View Article] [PubMed]
    [Google Scholar]
  41. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  42. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982 ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128, 19591968.
    [Google Scholar]
  43. Vreeland R. H., Litchfield C. D., Martin E. L., Elliot E. ( 1980 ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. . Int J Syst Bacteriol 30, 485495. [View Article]
    [Google Scholar]
  44. Wang Y., Cong Y., Liu J., Yuan Z., Huang C., Xu Q. ( 1998 ). Bacterial glycocalyx stain analyzed by light microscopical technique. . Chin J Lab Med 21, 197198.
    [Google Scholar]
  45. Wang Y.-N., Cai H., Yu S.-L., Wang Z.-Y., Liu J., Wu X.-L. ( 2007a ). Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil. . Int J Syst Evol Microbiol 57, 911915. [View Article] [PubMed]
    [Google Scholar]
  46. Wang Y.-N., Cai H., Chi C.-Q., Lu A.-H., Lin X.-G., Jiang Z.-F., Wu X.-L. ( 2007b ). Halomonas shengliensis sp. nov., a moderately halophilic, denitrifying, crude-oil-utilizing bacterium. . Int J Syst Evol Microbiol 57, 12221226. [View Article] [PubMed]
    [Google Scholar]
  47. Wang Y., Tang S.-K., Lou K., Mao P.-H., Jin X., Jiang C.-L., Xu L.-H., Li W.-J. ( 2008a ). Halomonas lutea sp. nov., a moderately halophilic bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 58, 20652069. [View Article] [PubMed]
    [Google Scholar]
  48. Wang Y., Wu Y.-H., Wang C.-S., Xu X.-W., Oren A., Zhu X.-F., Wu M. ( 2008b ). Halomonas salifodinae sp. nov., a halophilic bacterium isolated from a salt mine in China. . Int J Syst Evol Microbiol 58, 28552858. [View Article] [PubMed]
    [Google Scholar]
  49. Wang C.-Y., Wu S.-J., Ng C.-C., Tzeng W.-S., Shyu Y.-T. ( 2012 ). Halomonas beimenensis sp. nov., isolated from an abandoned saltern. . Int J Syst Evol Microbiol 62, 30133017. [View Article] [PubMed]
    [Google Scholar]
  50. Wu Y.-H., Xu X.-W., Huo Y.-Y., Zhou P., Zhu X.-F., Zhang H.-B., Wu M. ( 2008 ). Halomonas caseinilytica sp. nov., a halophilic bacterium isolated from a saline lake on the Qinghai-Tibet Plateau, China. . Int J Syst Evol Microbiol 58, 12591262. [View Article] [PubMed]
    [Google Scholar]
  51. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. ( 2007 ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. . Int J Syst Evol Microbiol 57, 16191624. [View Article] [PubMed]
    [Google Scholar]
  52. Xu X.-W., Wu Y.-H., Wang C.-S., Yang J.-Y., Oren A., Wu M. ( 2008 ). Marinobacter pelagius sp. nov., a moderately halophilic bacterium. . Int J Syst Evol Microbiol 58, 637640. [View Article] [PubMed]
    [Google Scholar]
  53. Yarza P., Ludwig W., Euzéby J., Amann R., Schleifer K.-H., Glöckner F. O., Rosselló-Móra R. ( 2010 ). Update of the all-species living tree project based on 16S and 23S rRNA sequence analyses. . Syst Appl Microbiol 33, 291299. [View Article] [PubMed]
    [Google Scholar]
  54. Zhao B., Wang H., Mao X., Li R., Zhang Y.-J., Tang S., Li W.-J. ( 2012 ). Halomonas xianhensis sp. nov., a moderately halophilic bacterium isolated from a saline soil contaminated with crude oil. . Int J Syst Evol Microbiol 62, 173178. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.051656-0
Loading
/content/journal/ijsem/10.1099/ijs.0.051656-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error