1887

Abstract

Strain S658 was isolated from a nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and gene showed that this strain clustered within the genus The closest related species was, in both cases, PETP02 with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658 differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S658( = LMG 27289 = CECT 8230).

Funding
This study was supported by the:
  • Programa de Desarrollo de Ciencas Básicas – Uruguay (PEDECIBA)
  • MICINN
  • JAE-Doc (CSIC)
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2014-03-01
2024-04-18
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