1887

Abstract

Urolithins are dibenzopyranone metabolites that exert anti-inflammatory activity and are produced by the gut microbiota from the dietary polyphenols ellagic acid (EA) and ellagitannins. However, the bacteria involved in this process remain unknown. We report here a novel bacterium, strain CEBAS 1/15P, capable of metabolizing EA to urolithins, that was isolated from healthy human faeces and characterized by determining phenotypic, biochemical and molecular methods. The strain was related to 7-10-1-b, the type and only reported strain of the only species of the genus with about 97 % 16S rRNA gene sequence similarity; they were both obligately anaerobic, non-spore-forming, Gram-stain-positive, short-rods/coccobacilli and metabolized only small numbers of carbon sources. -Fucose, -fructose, turanose, -galacturonic acid and α-ketobutyric acid were metabolized by strain CEBAS 1/15P, while was negative for metabolism of these compounds. The whole-cell fatty acids consisted predominantly of saturated fatty acids (70 %); strain CEBAS 1/15P differed significantly from in the major fatty acid, which was Cω9, while anteiso-C was the major component for . The presence of a number of different fatty acid peaks, especially C cyclo and Cω6, was also indicative of distinct species. Six glycolipids (GL1–6) were recognized, while, in only four glycolipids were described. On the basis of these data, the novel species sp. nov. is described, with strain CEBAS 1/15P ( = DSM 27213 = CCUG 64261) as the type strain.

Funding
This study was supported by the:
  • Fun-C-Food (Award Consolider Ingenio 2010 (project CSD2007-00063))
  • Fundación Séneca de la Región de Murcia (Award GERM 06, 04486)
  • CICYT (Award AGL2011-22447)
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2014-07-01
2024-03-28
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