1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_2

gen. nov., sp. nov., a member of the phylum isolated from Free

Abstract

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain RG1-1, was isolated from collected from Gawalong glacier in Tibet, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain RG1-1 were iso-C (19.8 %), summed feature 3 (Cω7 and/or Cω6, 17.0 %), C (9.9 %) and iso-C 3-OH (9.4 %); its major polar lipids were phosphatidylethanolamine, four unidentified aminolipids, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid, and three unidentified lipids. Strain RG1-1 contained MK-7 as the dominant menaquinone, and the G+C content of its genomic DNA was 49.1 mol%. Strain RG1-1 exhibited the highest 16S rRNA gene sequence similarity (91.8 %) with Gsoil 492 and Gsoil 643. Phylogenetic analysis showed that strain RG1-1 was a member of the family , phylum . On the basis of 16S rRNA gene sequence analysis, and phenotypic and chemotaxonomic data, strain RG1-1 is considered to represent a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is RG1-1 ( = CGMCC 1.12492 = DSM 26897).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31100004)
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.055749-0
2014-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/2/607.html?itemId=/content/journal/ijsem/10.1099/ijs.0.055749-0&mimeType=html&fmt=ahah

References

  1. An D.-S., Lee H.-G., Im W.-T., Liu Q. M., Lee S.-T. ( 2007 ). Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 57, 1828–1833. [View Article] [PubMed]
    [Google Scholar]
  2. Dong X.-Z., Cai M.-Y. ( 2001 ). Determination of biochemical properties. . In Mannual for Systematic Identification of General Bacteria, pp. 370–398. Edited by Dong X.-Z., Cai M.-Y. . Beijing:: Science Press; (in Chinese).
     [Google Scholar]
  3. Kämpfer P., Young C.-C., Sridhar K. R., Arun A. B., Lai W. A., Shen F. T., Rekha P. D. ( 2006 ). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov.. Int J Syst Evol Microbiol 56, 2223–2228. [View Article] [PubMed]
    [Google Scholar]
  4. Kämpfer P., Lodders N., Falsen E. ( 2011 ). Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.. Int J Syst Evol Microbiol 61, 518–523. [View Article] [PubMed]
    [Google Scholar]
  5. Kim B.-Y., Weon H.-Y., Yoo S.-H., Hong S.-B., Kwon S.-W., Stackebrandt E., Go S.-J. ( 2007 ). Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. . Int J Syst Evol Microbiol 57, 538–541. [View Article] [PubMed]
    [Google Scholar]
  6. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  7. Kroppenstedt R. M. ( 1982 ). Separation of bacterial menaquinones by HPLC using reverse phase (Rp18) and a silver loaded ion-exchanger as stationary phases. . J Liq Chromatogr 5, 2359–2367. [View Article]
    [Google Scholar]
  8. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
     [Google Scholar]
  9. Li Y. H., Liu Q. F., Liu Y., Zhu J. N., Zhang Q. ( 2011 ). Endophytic bacterial diversity in roots of Typha angustifolia L. in the constructed Beijing Cuihu Wetland (China). . Res Microbiol 162, 124–131. [View Article] [PubMed]
    [Google Scholar]
  10. Lim J. H., Baek S.-H., Lee S.-T. ( 2009 ). Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family ‘Chitinophagaceae’ in the phylum Bacteroidetes, isolated from freshwater sediment. . Int J Syst Evol Microbiol 59, 2394–2399. [View Article] [PubMed]
    [Google Scholar]
  11. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  12. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  13. Minnikin D. E., Odonnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  14. Qu J.-H., Yuan H.-L. ( 2008 ). Sediminibacterium salmoneum gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir. . Int J Syst Evol Microbiol 58, 2191–2194. [View Article] [PubMed]
    [Google Scholar]
  15. Qu J.-H., Yuan H.-L., Yang J.-S., Li H.-F., Chen N. ( 2009 ). Lacibacter cauensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from sediment of a eutrophic lake. . Int J Syst Evol Microbiol 59, 1153–1157. [View Article] [PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria gas chromatography of cellular fatty acids. . MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  18. Shiratori H., Tagami Y., Morishita T., Kamihara Y., Beppu T., Ueda K. ( 2009 ). Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. . Int J Syst Evol Microbiol 59, 1137–1142. [View Article] [PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  21. Wang Y., Cai F., Tang Y., Dai J., Qi H., Rahman E., Peng F., Fang C. ( 2011 ). Flavitalea populi gen. nov., sp. nov., isolated from soil of a Euphrates poplar (Populus euphratica) forest. . Int. J. Syst. Evol. Microbiol. 61, 1554–1560. [View Article] [PubMed]
    [Google Scholar]
  22. Weon H.-Y., Kim B.-Y., Yoo S.-H., Lee S.-Y., Kwon S.-W., Go S.-J., Stackebrandt E. ( 2006 ). Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. . Int J Syst Evol Microbiol 56, 1777–1782. [View Article] [PubMed]
    [Google Scholar]
  23. Xie C. H., Yokota A. ( 2006 ). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56, 1117–1121. [View Article] [PubMed]
    [Google Scholar]
  24. Yoon M.-H., Im W.-T. ( 2007 ). Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. . Int J Syst Evol Microbiol 57, 1834–1839. [View Article] [PubMed]
    [Google Scholar]
  25. Zhang K., Tang Y., Zhang L., Dai J., Wang Y., Luo X., Liu M., Luo G., Fang C. ( 2009 ). Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil. . Int J Syst Evol Microbiol 59, 3058–3062. [View Article] [PubMed]
    [Google Scholar]
  26. Zhang N. N., Qu J. H., Yuan H. L., Sun Y. M., Yang J. S. ( 2010a ). Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest. . Int J Syst Evol Microbiol 60, 1609–1612. [View Article] [PubMed]
    [Google Scholar]
  27. Zhang K., Wang Y., Tang Y., Dai J., Zhang L., An H., Luo G., Rahman E., Fang C. ( 2010b ). Niastella populi sp. nov., isolated from soil of Euphrates poplar (Populus euphratica) forest, and emended description of the genus Niastella . . Int J Syst Evol Microbiol 60, 542–545. [View Article] [PubMed]
    [Google Scholar]
  28. Zhang J., Gu T., Zhou Y., He J., Zheng L.-Q., Li W.-J., Huang X., Li S.-P. ( 2012 ). Terrimonas rubra sp. nov., isolated from a polluted farmland soil and emended description of the genus Terrimonas . . Int J Syst Evol Microbiol 62, 2593–2597. [View Article] [PubMed]
    [Google Scholar]
  29. Zhang L., Qin B., Jia Z., Wei G. ( 2013 ). Flavitalea gansuensis sp. nov., isolated from soil from an arid area, and emended descriptions of the genus Flavitalea and Flavitalea populi . . Int J Syst Evol Microbiol 63, 490–495. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.055749-0
Loading
Cnuella takakiae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides
Int J Syst Evol Microbiol 64, 607 (2014); https://doi.org/10.1099/ijs.0.055749-0
/content/journal/ijsem/10.1099/ijs.0.055749-0
/content/journal/ijsem/10.1099/ijs.0.055749-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error