1887

Abstract

A facultatively alkaliphilic, lactic-acid-producing and halophilic strain, designated SG103, was isolated from a fermented indigo ( Lour.) liquor sample for dyeing prepared in a laboratory. 16S rRNA gene sequence phylogeny suggested that SG103 is a member of the genus with the closest relatives being ‘ ’ J2 (similarity: 97.06 %), TP2-8 (97.06 %) and NN (96.87 %). Cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. The strain grew at temperatures between 13 and 48 °C with the optimum at 39 °C. It grew in the range pH 7–10 with the optimum at pH 9. The isoprenoid quinone detected was menaquinone-7 (MK-7) and the DNA G+C content was 41.3 mol%. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C, anteiso-C and anteiso-C. Unlike other reported species of the genus , the strain lacked diphosphatidylglycerol as a major polar lipid. DNA–DNA hybridization experiments with strains exhibiting greater than 96.87 % 16S rRNA gene sequence similarity, ‘. ’ J2, TP2-8 and NN, revealed 2±4 %, 4±9 % and 3±2 % relatedness, respectively. On the basis of the differences in phenotypic and chemotaxonomic characteristics, and the results of phylogenetic analyses based on 16S rRNA gene sequences and DNA–DNA relatedness data from reported species of the genus , strain SG103 merits classification as a members of a novel species, for which the name sp. nov. is proposed. The type strain is SG103 ( = JCM 17253 = NCIMB 14683).

Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 23570128)
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2014-09-01
2024-03-29
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