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Volume 64, Issue Pt_8

sp. nov. and sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Free

Abstract

Three strictly anaerobic, Gram-positive, non-spore-forming, rod-shaped, motile bacteria, designated strains ACB1, ACB7 and ACB8, were isolated from human subgingival dental plaque. All strains required yeast extract for growth. Strains ACB1 and ACB8 were able to grow on glucose, lactose, maltose, maltodextrin and raffinose; strain ACB7 grew weakly on sucrose only. The growth temperature range was 30–42 °C with optimum growth at 37 °C. Major metabolic fermentation end products of strain ACB1 were acetate and lactate; the only product of strains ACB7 and ACB8 was acetate. Major fatty acids of strain ACB1 were C, C, Cω7 dimethyl aldehyde (DMA) and Cω7 DMA. Major fatty acids of strain ACB7 were C, C, C, Cω7 and Cω7 DMA. The hydrolysate of the peptidoglycan contained -diaminopimelic acid, indicating peptidoglycan type A1γ. Genomic DNA G+C content varied from 42 to 43.3 % between strains. According to 16S rRNA gene sequence phylogeny, strains ACB1, ACB8 and ACB7 formed two separate branches within the genus , with 98.1–98.6 % sequence similarity to the type strain of the type species, . Predicted DNA–DNA hybridization values between strains ACB1, ACB8, ACB7 and F0268 were <70 %. Based on distinct genotypic and phenotypic characteristics, strains ACB1 and ACB8, and strain ACB7 are considered to represent two distinct species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains are ACB1 ( = DSM 24637 = HM-481 = ATCC BAA-2638) and ACB7 ( = DSM 24638 = HM-482 = ATCC BAA-2639), respectively.

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Funding
This study was supported by the:
  • NIH (Award EF-1002148, 1U54 AI84844-01, 3 R21 DE018026-02S1 and 1RC1DE020707-01)
  • EU-FP7 Marie Curie International (Award U54HG004969 and PIOF-GA-2009-235470)
© 2014 IUMS
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2014-08-01
2024-04-19
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Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium
Int J Syst Evol Microbiol 64, 2642 (2014); https://doi.org/10.1099/ijs.0.060988-0
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