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Volume 64, Issue Pt_8

gen. nov., sp. nov., a benzoate-oxidizing, sulfate-reducing bacterium isolated from water extracted from a coal bed Free

Abstract

A novel, strictly anaerobic, sulfate-reducing bacterium, designated strain SCBM, was isolated from water extracted from a coal bed in Indiana, USA. The isolate was characterized by a polyphasic taxonomic approach that included phenotypic and genotypic characterizations. Cells of strain SCBM were vibrio-shaped, polarly flagellated, Gram-negative, motile, oxidase-negative and weakly catalase-positive. Growth of strain SCBM was observed at NaCl concentrations ranging from 0 to 300 mM. However, no growth was observed when 1 M or more NaCl was present. Growth was observed at 16–37 °C, with optimal growth at 30 °C. The optimum pH for growth was 7, although growth was observed from pH 6.5 to 8. The doubling time under optimal growth conditions (30 °C, pH 7, 2.5 mM benzoate, 14 mM sulfate) was 2.7 days. Bicarbonate, HEPES, PIPES and MES were effective buffers for growth of strain SCBM, but citrate inhibited growth. When sulfate was provided as the electron acceptor, strain SCBM grew autotrophically with hydrogen as the electron donor and heterotrophically on benzoate, formate, acetate, pyruvate, butyrate, fumarate, succinate and palmitate. None of the substrates tested supported fermentative growth. Thiosulfate and sulfate were used as electron acceptors coupled to benzoate oxidation, but sulfite, elemental sulfur, DMSO, anthraquinone 2,6-disulfonate, nitrate, nitrite, ferric citrate, hydrous iron oxide and oxygen were not. The G+C content of genomic DNA was 62.5 mol%. The major cellular fatty acids were -C and Cω7. Phylogenetic analysis based on 16S rRNA gene sequencing placed strain SCBM into a distinct lineage within the class . The closest, cultivated phylogenetic relative of strain SCBM was DSM 2075, with only 91.7 % 16S rRNA gene sequence identity. On the basis of phenotypic and genotypic analyses, strain SCBM represents a novel genus and species of sulfate-reducing bacteria, for which the name gen. nov., sp. nov. is proposed. The type strain of is SCBM ( = DSM 28127 = JCM 19826). is the second genus of the order .

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Funding
This study was supported by the:
  • School of Public and Environmental Affairs, Indiana University
  • Vietnam Education Foundation
© 2014 IUMS
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2014-08-01
2024-04-20
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Desulfocarbo indianensis gen. nov., sp. nov., a benzoate-oxidizing, sulfate-reducing bacterium isolated from water extracted from a coal bed
Int J Syst Evol Microbiol 64, 2907 (2014); https://doi.org/10.1099/ijs.0.064873-0
/content/journal/ijsem/10.1099/ijs.0.064873-0
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