1887

Abstract

Four staphylococcal isolates from clinical and necropsy specimens from a cat, a dog, a horse and a parrot () were found to constitute a distinct taxon. 16S rRNA gene sequence analysis revealed that its closest phylogenetic relatives are and . Growth characteristics, biochemical features and DNA–DNA hybridizations demonstrated that the strains differ from these and other known species and that they represent a single, novel species for which the name sp. nov. is proposed. The novel species is commonly confused with in routine diagnostic veterinary bacteriology. Although the strains described were isolated from lesions and show several characteristics typical of pathogenic staphylococci, such as coagulase, DNase and -haemolysin production, the pathogenic significance of the novel species remains unclear. The type strain, LMG 22219 (=ON 86=CCUG 49543), was isolated from lung tissue of a cat.

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2005-07-01
2024-03-28
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References

  1. Baele M., Baele P., Vaneechoutte M., Storms V., Butaye P., Devriese L. A., Verschraegen G., Gillis M., Haesebrouck F. 2000; Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38:4201–4207
    [Google Scholar]
  2. Baele M., Storms V., Haesebrouck F., Devriese L. A., Gillis M., Verschraegen G., De Baere T., Vaneechoutte M. 2001; Application and evaluation of the interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) for identification of Streptococcus species. J Clin Microbiol 39:1436–1442 [CrossRef]
    [Google Scholar]
  3. Calvo J., Hernandez J. L., Farinas M. C., Garcia-Palomo D., Aguero J. 2000; Osteomyelitis caused by Staphylococcus schleiferi and evidence of misidentification of this Staphylococcus species by an automated bacterial identification system. J Clin Microbiol 38:3887–3889
    [Google Scholar]
  4. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P. 1999; Classification of Alcaligenes faecalis -like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49:405–413 [CrossRef]
    [Google Scholar]
  5. De La Fuente R., Suarez G., Schleifer K. H. 1985; Staphylococcus aureus subsp. anaerobius subsp. nov. the causal agent of abscess disease of sheep. Int J Syst Bacteriol 35:99–102 [CrossRef]
    [Google Scholar]
  6. Devriese L. A. 1981; Baird-Parker medium supplemented with acriflavine, polymyxins and sulphonamide for the selective isolation of Staphylococcus aureus from heavily contaminated materials. J Appl Bacteriol 50:351–357 [CrossRef]
    [Google Scholar]
  7. Devriese L. A., Hajek V. 1980; Identification of pathogenic staphylococci isolated from animals and foods derived from animals. J Appl Bacteriol 49:1–11 [CrossRef]
    [Google Scholar]
  8. Devriese L. A., Van de Kerckhove A. 1979; A comparison of methods and the validity of deoxyribonuclease tests for the characterization of staphylococci isolated from animals. J Appl Bacteriol 46:385–393 [CrossRef]
    [Google Scholar]
  9. Devriese L. A., Hajek V., Oeding P., Meyer S., Schleifer K. H. 1978; Staphylococcus hyicus (Sompolinsky 1953) comb. nov. and Staphylococcushyicus subsp. chromogenes subsp. nov. Int J Syst Bacteriol 28:482–490 [CrossRef]
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  11. Foster G., Ross H. M., Hutson R. A., Collins M. D. 1997; Staphylococcus lutrae sp. nov., a new coagulase-positive species isolated from otters. Int J Syst Bacteriol 47:724–726 [CrossRef]
    [Google Scholar]
  12. Freney J., Kloos W. E., Hajek V., Webster J. A., Bes M., Brun Y., Vernozy-Rozand C. 1999; Recommended minimal standards for description of new staphylococcal species. Int J Syst Bacteriol 49:489–502 [CrossRef]
    [Google Scholar]
  13. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. 1998; Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [CrossRef]
    [Google Scholar]
  14. Hajek V. 1976; Staphylococcus intermedius , a new species isolated from animals. Int J Syst Bacteriol 26:401–408 [CrossRef]
    [Google Scholar]
  15. Hébert G. A., Crowder C. G., Hancock G. A., Jarvis W. R., Thornsberry C. 1988; Characteristics of coagulase-negative staphylococci that help differentiate these species and other members of the family Micrococcaceae . J Clin Microbiol 26:1939–1949
    [Google Scholar]
  16. Igimi S., Takahashi E., Mitsuoka T. 1990; Staphylococcus schleiferi subsp. coagulans subsp. nov., isolated from the external auditory meatus of dogs with external ear otitis. Int J Syst Bacteriol 40:409–411 [CrossRef]
    [Google Scholar]
  17. Isigidi B. K., Devriese L. A., Croegaert T., Van Hoof J. 1989; A highly selective two-stage-enumeration method for the selective enumeration of Staphylococcus aureus in foods. J Appl Bacteriol 66:379–384 [CrossRef]
    [Google Scholar]
  18. Kloos W. E., Bannerman T. L. 1999; Staphylococcus and Micrococcus . In Manual of Clinical Microbiology , 7th edn. pp  264–282 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  20. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  21. Takahashi E. T., Satoh I., Kikuchi N. 1999; Phylogenetic relationships of 38 taxa of the genus Staphylococcus based on 16S rRNA gene sequence analysis. Int J Syst Bacteriol 49:725–728 [CrossRef]
    [Google Scholar]
  22. Vandenesch F., Labeau C., Bes M. 7 other authors 1994; Clotting activity in Staphylococcus schleiferi subspecies from human patients. J Clin Microbiol 32:388–392
    [Google Scholar]
  23. Varaldo P. E., Kilpper-Bälz R., Biavasco F., Satta G., Schleifer K. H. 1988; Staphylococcus delphini sp. nov., a coagulase-positive species isolated from dolphins. Int J Syst Bacteriol 38:436–439 [CrossRef]
    [Google Scholar]
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