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Volume 55, Issue 5

Phylogeny of the based on genes encoding elongation factor Tu and F-ATPase -subunit Free

Abstract

The phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor Tu gene () and of their F-ATPase -subunit gene (). An 884 bp fragment for and an 884 or 871 bp fragment for were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. The sequence analysis exhibited an indel specific to and species, showing, for the first time, a tight phylogenetic affiliation between these two genera. Comprehensive and phylogenetic trees were constructed and are in agreement with each other. Monophyletic genera are , , , , , , and . Analogous trees based on 16S rRNA gene sequences available from databases were also reconstructed. The and phylogenies are in agreement with the 16S rRNA gene sequence analysis, and distance comparisons revealed that the and genes provide better discrimination for pairs of species belonging to the family . In conclusion, phylogeny based on and conserved genes allows discrimination between species of the .

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2005-09-01
2024-04-18
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Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit
Int J Syst Evol Microbiol 55, 2013 (2005); https://doi.org/10.1099/ijs.0.63539-0
/content/journal/ijsem/10.1099/ijs.0.63539-0
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