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Volume 55, Issue 5

gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage ( ord. nov.) in the ‘’ Free

Abstract

A marine bacterium, designated strain GW14-5, capable of degrading high-molecular-mass polycyclic aromatic hydrocarbons was isolated from the sediments of Gwangyang Bay, Republic of Korea, after enrichment culture for 2 years with a mixture of benzo[a]pyrene and pyrene. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate forms a phyletic lineage that is distinct from the seven known orders within the ‘’. 16S rRNA gene sequence similarity of strain GW14-5 to all recognized bacterial species was not greater than 92 %. The dominant fatty acids of the isolate were i-17 : 1 (46·2 %), i-15 : 0 (15·1 %) and i-17 : 0 (12·6 %). The major respiratory quinone was MK-5, and the DNA G+C content was 39·3 mol%. Cells of strain GW14-5 were Gram-negative, motile, catalase-positive, oxidase-positive and weakly halophilic. Glucose, -acetylglucosamine and maltose were utilized as sole carbon sources. The strain was positive for -glucosidase activity. Optimal growth of strain GW14-5 was at pH 7·0 and 37–40 °C and required the presence of 2 % (w/v) NaCl. On the basis of this evidence, strain GW14-5 represents a novel genus and species in the ‘’ for which the name gen. nov., sp. nov. is proposed. The novel order is proposed for the distinct phyletic line represented by the genus . The type strain is GW14-5 (=KCCM 42021=JCM 12864).

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Keyword(s): BaP, benzo[a]pyrene and PAH, polycyclic aromatic hydrocarbon
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2005-09-01
2024-04-20
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References

  1. Bae S. S., Lee J.-H., Kim S.-J. 2005; Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 55:1211–1215 [CrossRef]
     [Google Scholar]
  2. Cho J.-C., Giovannoni S. J. 2003; Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the Alphaproteobacteria . Int J Syst Evol Microbiol 53:1031–1036 [CrossRef]
     [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analysis in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  4. Garrity G. M., Holt J. G. 2001; The road map to the Manual. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  119–166 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
     [Google Scholar]
  5. Jordan D. C. 1984; Genus I. Rhizobium Frank 1889, 338AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp  235–242 Edited by Krieg N. L., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  6. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  7. Kocur M. 1984; Genus Paracoccus Davis 1969, 384AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp  399–402 Edited by Krieg N. L., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  8. Krieg N. R., Döbereiner J. 1984; Genus Azospirillum Tarrand, Krieg and Döbereiner 1979, 79AL (effective publication: Tarrand, Krieg and Döbereiner 1978, 978. In Bergey's Manual of Systematic Bacteriology vol 1 pp  94–104 Edited by Krieg N. L., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  9. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. 1999; Roseovarius tolerans gen. nov., sp. nov. a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49:137–147 [CrossRef]
     [Google Scholar]
  10. Lee J. H., Kim Y. S., Choi T. J., Lee W. J., Kim Y. T. 2004; Paracoccus haeundensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. Int J Syst Evol Microbiol 54:1699–1702 [CrossRef]
     [Google Scholar]
  11. Mergaert J., Cnockaert M. C., Swings J. 2002; Phyllobacterium myrsinacearum (subjective synonym Phyllobacterium rubiacearum ) emend. Int J Syst Evol Microbiol 52:1821–1823 [CrossRef]
     [Google Scholar]
  12. Poindexter J. S. 1989; Genus Caulobacter Henrici and Johnson 1935, 83AL . In Bergey's Manual of Systematic Bacteriology vol. 3 pp  1924–1939 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  14. Schleheck D., Tindall B. J., Rossello-Mora R., Cook A. M. 2004; Parvibaculum lavamentivorans gen. nov., sp. nov. a novel heterotroph that initiates catabolism of linear alkylbenzenesulfonate. Int J Syst Evol Microbiol 54:1489–1497 [CrossRef]
     [Google Scholar]
  15. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria Baltimore: Williams & Wilkins;
     [Google Scholar]
  16. Sohn J. H., Kwon K. K., Kang J.-H., Jung H.-B., Kim S.-J. 2004; Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54:1483–1487 [CrossRef]
     [Google Scholar]
  17. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp  158–160 Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
     [Google Scholar]
  18. Swings J., De Ley J. 1984; Genus Zymomonas Kluyver and van Niel, 1936, 399AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  576–580 Edited by Krieg N. L., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  19. Weiss E., Moulder J. W. 1984; Genus I. Rickettsia da Rocha-Lima 1916, 567AL (Nom. gen. cons. Opin.) 19, Jud Comm 1958, 158. In Bergey's Manual of Systematic Bacteriology vol 1 pp  688–698 Edited by Krieg N. L., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  20. Zhang Y., Zhu Y.-X., Kwon K. K., Park J. H., Kim S.-J. 2004; Novel method for determining pyrene biodegradation using synchronous fluorimetry. Chemosphere 55:389–394 [CrossRef]
     [Google Scholar]
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Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’
Int J Syst Evol Microbiol 55, 2033 (2005); https://doi.org/10.1099/ijs.0.63684-0
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