1887

Abstract

Fifteen bacterial strains isolated from molasses grass ( Beauv.) were identified as nitrogen-fixers by using the acetylene-reduction assay and PCR amplification of gene fragments. These strains were classified as a unique group by insertion sequence-PCR fingerprinting, SDS-PAGE protein patterns, DNA–DNA hybridization, 16S rRNA gene sequencing and morphological characterization. Phylogenetic analysis of the 16S rRNA gene indicated that these diazotrophic strains belonged to the genus and were closely related to (with 97.5 % similarity). In all the analyses, including in addition phenotypic characterization using Biolog MicroPlates and comparison of cellular fatty acids, this novel group was found to be different from the most closely related species, . Based on these data, a novel species, sp. nov., is proposed for these endophytic diazotrophs of , with TMCY 0552 (=CCBAU 5106001=LMG 23364=CGMCC 1.5340) as the type strain.

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2006-06-01
2024-03-28
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References

  1. Adhikari T. B., Mew T. W., Leach J. E. 1999; Genotypic and pathotypic diversity in Xanthomonas oryzae pv. oryzae in Nepal. Phytopathology 89:687–694 [CrossRef]
    [Google Scholar]
  2. Baldani J. I., Baldani V. L. D., Seldin L., Döbereiner J. 1986; Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. Int J Syst Bacteriol 36:86–93 [CrossRef]
    [Google Scholar]
  3. Baldani J. I., Caruso L., Baldani V. L. D., Goi S. R., Döbereiner J. 1997; Recent advances in BNF with non-legume plants. Soil Biol Biochem 29:911–922 [CrossRef]
    [Google Scholar]
  4. Bashan Y., Holguin G. 1997; Azospirillum -plant relationships: environmental and physiological advances (1990–1996. Can J Microbiol 43:103–121 [CrossRef]
    [Google Scholar]
  5. Ben Dekhil S., Cahill M., Stackebrandt E., Sly L. I. 1997; Transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum as Azospirillum largomobile comb. nov., and elevation of Conglomeromonas largomobilis subsp. parooensis to the new type species of Conglomeromonas , Conglomeromonas parooensis sp. nov. Syst Appl Microbiol 20:72–77 [CrossRef]
    [Google Scholar]
  6. Boddey R. M., Urquiaga S., Alves B. J. R., Reis V. 2003; Endophytic nitrogen fixation in sugarcane: present knowledge and future applications. Plant Soil 252:139–149 [CrossRef]
    [Google Scholar]
  7. Bruce R. A., Achenbach L. A., Coates J. D. 1999; Reduction of (per)chlorate by a novel organism isolated from paper mill waste. Environ Microbiol 1:319–329 [CrossRef]
    [Google Scholar]
  8. Burris R. H. 1972; Nitrogen fixation – assay methods and techniques. Methods Enzymol 24B:415–431
    [Google Scholar]
  9. Cavalcante V. A., Döbereiner J. 1988; A new acid-tolerant nitrogen-fixing bacterium associated with sugarcane. Plant Soil 108:23–32 [CrossRef]
    [Google Scholar]
  10. Coates J. D., Michaelidou U., Bruce R. A., O'Connor S. M., Crespi J. N., Achenbach L. A. 1999; Ubiquity and diversity of dissimilatory (per)chlorate-reducing bacteria. Appl Environ Microbiol 65:5234–5241
    [Google Scholar]
  11. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  12. Döbereiner J. 1980; Forage grasses and grain crops. In Methods for Evaluation of Biological Nitrogen Fixation pp  535–555 Edited by Bergersen J. F. J. Chichester: Wiley;
    [Google Scholar]
  13. Döbereiner J. 1983; Ten years of Azospirillum . In Azospirillum II. Genetics , Physiology , Ecology pp  9–23 Edited by Klingmueller W. Basel: Birkhaeuser;
    [Google Scholar]
  14. Eckert B., Weber O. B., Kirchhof G., Halbritter A., Stoffels M., Hartmann A. 2001; Azospirillum doebereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass Miscanthus . Int J Syst Evol Microbiol 51:17–26
    [Google Scholar]
  15. Engelhard M., Hurek T., Reinhold-Hurek B. 2000; Preferential occurrence of diazotrophic endophytes, Azoarcus spp., in wild rice species and land races of Oryza sativa in comparison with modern races. Environ Microbiol 2:131–141 [CrossRef]
    [Google Scholar]
  16. Hallmann J., Quadt-Hallmann A., Mahaffee W. F., Kloepper J. W. 1997; Bacterial endophytes in agricultural crops. Can J Microbiol 43:895–914 [CrossRef]
    [Google Scholar]
  17. Hurek T., Egener T., Reinhold-Hurek B. 1997; Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the β subclass. J Bacteriol 179:4172–4178
    [Google Scholar]
  18. Khammas K. M., Ageron E., Grimont P. A. D., Kaiser P. 1989; Azospirillum irakense sp. nov., a new nitrogen-fixing bacterium associated with rice roots and rhizosphere soil. Res Microbiol 140:679–693
    [Google Scholar]
  19. Khan Z. R., Ampong-Nyarko K., Chiliswa P. 8 other authors 1997; Intercropping increases parasitism of pests. Nature 388:631–632
    [Google Scholar]
  20. Kirchhof G., Reis V. M., Baldani J. I., Eckert B., Döbereiner J., Hartmann A. 1997; Occurrence, physiological and molecular analysis of endophytic diazotrophic bacteria in gramineous energy plants. Plant Soil 194:45–55 [CrossRef]
    [Google Scholar]
  21. Krieg N. R., Döbereiner J. 1984; Genus Azospirillum Tarrand, Krieg and Döbereiner 1979, 79AL (effective publication: Tarrand, Krieg and Döbereiner 1978, 978. In Bergey's Manual of Systematic Bacteriology vol 1 pp  94–104 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  22. Magalhães F. M., Baldani J. I., Souto S. M., Kuykendall J. R., Döbereiner J. 1983; A new acid-tolerant Azospirillum species. Ann Acad Bras Cienc 55:417–430
    [Google Scholar]
  23. Maidak B. L., Cole J. R., Parker C. T. Jr 11 other authors 1999; A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  24. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  25. Martínez L., Caballero-Mellado J., Orozco J., Martínez-Romero E. 2003; Diazotrophic bacteria associated with banana ( Musa spp.). Plant Soil 257:35–47 [CrossRef]
    [Google Scholar]
  26. McInroy J. A., Kloepper J. W. 1995; Population dynamics of endophytic bacteria in field-grown sweet corn and cotton. Can J Microbiol 41:895–901 [CrossRef]
    [Google Scholar]
  27. Okon Y. 1985; Azospirillum as a potential inoculant for agriculture. Trends Biotechnol 3:223–228 [CrossRef]
    [Google Scholar]
  28. Olivares F. L., Baldani V. L. D., Reis V. M., Baldani J. I., Dobereiner J. 1996; Occurrence of the endophytic diazotrophs Herbaspirillum spp. in roots, stems, and leaves, predominantly of Gramineae. Biol Fertil Soils 21:197–200 [CrossRef]
    [Google Scholar]
  29. Reinhold B., Hurek T. 1988; Location of diazotrophs in the root interior with special attention to the Kallar grass association. Plant Soil 110:259–268 [CrossRef]
    [Google Scholar]
  30. Reinhold B., Hurek T., Niemann E.-G., Fendrik I. 1986; Close association of Azospirillum and diazotrophic rods with different root zones of Kallar grass. Applied Environ Microbiol 52:520–526
    [Google Scholar]
  31. Reinhold B., Hurek T., Fendrik I., Pot B., Gillis M., Kersters K., Thielemans S., De Ley J. 1987; Azospirillum halopraeferens sp. nov., a nitrogen-fixing organism associated with roots of Kallar grass ( Leptochloa fusca (L.) Kunth). Int J Syst Bacteriol 37:43–51 [CrossRef]
    [Google Scholar]
  32. Reinhold-Hurek B., Hurek T. 2000; Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen.nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov. Int J Syst Evol Microbiol 50:649–659 [CrossRef]
    [Google Scholar]
  33. Reinhold-Hurek B., Hurek T., Gillis M., Hoste B., Vancanneyt M., Kersters K., De Ley J. 1993; Azoarcus gen. nov., nitrogen-fixing proteobacteria associated with roots of Kallar grass ( Leptochloa fusca (L.) Kunth) and description of two species, Azoarcus indigens sp.nov. and Azoarcus communis sp. nov. Int J Syst Bacteriol 43:574–584 [CrossRef]
    [Google Scholar]
  34. Reis V. M., Estrada-de los Santos P., Tenorio-Salgado S. 10 other authors 2004; Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium. Int J Syst Evol Microbiol 54:2155–2162 [CrossRef]
    [Google Scholar]
  35. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  36. Salles J. F., Gitahy P. D. M., Skot L., Baldani J. I. 2000; Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis . Braz J Microbiol 31:154–160 [CrossRef]
    [Google Scholar]
  37. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  38. Schenk S. U., Werner D. 1988; Fatty acid analysis of four Azospirillum species reveals three groups. Arch Microbiol 149:580–582 [CrossRef]
    [Google Scholar]
  39. Sly L. I., Stackebrandt E. 1999; Description of Skermanella parooensis gen. nov., sp. nov.to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum . Int J Syst Bacteriol 49:541–544 [CrossRef]
    [Google Scholar]
  40. Tan Z., Reinhold-Hurek B. 2003; Dechlorosoma suillum Achenbach et al . 2001 is a later subjective synonym of Azospira oryzae Reinhold-Hurek and Hurek 2000. Int J Syst Evol Microbiol 53:1139–1142 [CrossRef]
    [Google Scholar]
  41. Tan Z., Hurek T., Gyaneshwar P., Ladha J. K., Reinhold-Hurek B. 2001a; Novel endophytes of rice form a taxonomically distinct subgroup of Serratia marcescens . Syst Appl Microbiol 24:245–251 [CrossRef]
    [Google Scholar]
  42. Tan Z., Hurek T., Vinuesa P., Müller P., Ladha J. K., Reinhold-Hurek B. 2001b; Specific detection of Bradyrhizobium and Rhizobium strains colonizing rice ( Oryza sativa ) roots by 16S-23S ribosomal DNA intergenic spacer-targeted PCR. Appl Environ Microbiol 67:3655–3664 [CrossRef]
    [Google Scholar]
  43. Tan Z. Y., Kan F. L., Peng G. X., Wang E. T., Reinhold-Hurek B., Chen W. X. 2001c; Rhizobium yanglingense sp. nov., isolated from arid and semi-arid regions in China. Int J Syst Evol Microbiol 51:909–914 [CrossRef]
    [Google Scholar]
  44. Tarrand J. J., Krieg N. R., Döbereiner J. 1978; A taxonomic study of the Spirillum lipoferum group, with description of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol 24:967–980 [CrossRef]
    [Google Scholar]
  45. Thompson K. C., Roa E. J., Romero N. T. 1978; Anti-tick grasses as the basis for developing practical tropical tick control packages. Trop Anim Health Prod 10:179–182 [CrossRef]
    [Google Scholar]
  46. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  47. Vessey J. K. 2003; Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586 [CrossRef]
    [Google Scholar]
  48. Weiner M., Dacko J., Osek J. 2004; Insertion element IS3 PCR-based method for molecular analysis of enterotoxigenic Escherichia coli isolated from sucking piglets with diarrhoea. Bull Vet Inst Pulawy 48:241–246
    [Google Scholar]
  49. Xie C.-H., Yokota A. 2005; Azospirillum oryzae sp. nov., a nitrogen-fixing bacterium isolated from the roots of the rice plant Oryza sativa . Int J Syst Evol Microbiol 55:1435–1438 [CrossRef]
    [Google Scholar]
  50. Zehr J. P., McReynolds L. A. 1989; Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii . Appl Environ Microbiol 55:2522–2526
    [Google Scholar]
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