1887

Abstract

Two previously undescribed anamorphic yeasts, strains T-11 and T-26, recovered from wild rabbit faecal pellets collected in Muju, Korea, were identified using phenotypic and molecular taxonomic methods. The isolates were characterized by the proliferation of budding cells, positive diazonium blue B and urease reactions, the presence of Q-10 as the major ubiquinone, the presence of xylose in whole-cell hydrolysates and the inability to ferment sugars. Phylogenetic analyses based on 26S rRNA gene partial sequences revealed that strain T-11 was located in the clade and was related to , , and . Strain T-26 was located in the Mesenterica clade and was closely related to sp. F6 and CBS 8976. Sequence divergence values of more than 4 % from other described species, together with the phenotypic differences, showed that the isolated yeasts represent previously unrecognized members of this genus. Therefore, two novel yeast species are proposed: sp. nov., with strain T-11 (=KCTC 17231=CBS 10308) as the type strain, and sp. nov., with strain T-26 (=KCTC 17232=CBS 10309) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64353-0
2006-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/9/2241.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64353-0&mimeType=html&fmt=ahah

References

  1. Fell J. W., Statzell-Tallman A. 1998; Cryptococcus Vuillemin. In The Yeasts , a Taxonomic Study . , 4th edn. pp  742–767 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
  2. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1995 phylip (phylogeny inference package), version 3.57c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  5. Guarner F., Schaafsma G. J. 1998; Probiotics. Int J Food Microbiol 39:237–238 [CrossRef]
    [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–206
    [Google Scholar]
  7. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  8. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  9. Yamada Y., Kondo K. 1973; Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus , and the yeast-like genera Sporobolomyces and Rhodosporidium . J Gen Appl Microbiol 19:59–77 [CrossRef]
    [Google Scholar]
  10. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study , 4th edn. pp  77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64353-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64353-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error