1887

Abstract

A polyphasic study was performed to determine the taxonomic position of two strains, 665N and 868E, isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus . Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E clustered together with a similarity of 78 % but did not cluster with any of the genomospecies. DNA–DNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related genomospecies (30–44 %). Useful tests for the phenotypic differentiation of strains 665N and 868E from other mesophilic species included those for gas from glucose, lysine decarboxylase, Voges–Proskauer reaction, acid from -arabinose, hydrolysis of aesculin and utilization of -lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868E are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 868E (=CECT 7113=LMG 23376).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64497-0
2007-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/3/582.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64497-0&mimeType=html&fmt=ahah

References

  1. Abbott S. L., Cheung W. K. W., Janda J. M. 2003; The genus Aeromonas : biochemical characteristics, atypical reactions, and phenotypic identification schemes. J Clin Microbiol 41:2348–2357 [CrossRef]
    [Google Scholar]
  2. Altwegg M., Steigerwalt A. G., Altwegg-Bissig R., Lüthy-Hottenstein J., Brenner D. J. 1990; Biochemical identification of Aeromonas genospecies isolated from humans. J Clin Microbiol 28:258–264
    [Google Scholar]
  3. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  4. Carson J., Wagner T., Wilson T., Donachie L. 2001; Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix. J Appl Microbiol 90:190–200 [CrossRef]
    [Google Scholar]
  5. Esteve C., Valera L., Gutiérrez C., Ventosa A. 2003; Taxonomic study of sucrose-positive Aeromonas jandaei -like isolates from faeces, water and eels: emendation of A. jandaei Carnahan et al. 1992. Int J Syst Evol Microbiol 53:1411–1419 [CrossRef]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Gevers D., Huys G., Swings J. 2001; Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31–36 [CrossRef]
    [Google Scholar]
  8. Harf-Monteil C., Le Flèche A., Riegel P., Prévost G., Bermond D., Grimont P. A. D., Monteil H. 2004; Aeromonas simiae sp. nov., isolated from monkey faeces. Int J Syst Evol Microbiol 54:481–485 [CrossRef]
    [Google Scholar]
  9. Huys G., Swings J. 1999; Evaluation of a fluorescent amplified fragment length polymorphism (FAFLP) methodology for the genotypic discrimination of Aeromonas taxa. FEMS Microbiol Lett 177:83–92 [CrossRef]
    [Google Scholar]
  10. Huys G., Kämpfer P., Altwegg M., Coopman R., Janssen P., Gillis M., Kersters K. 1997; Inclusion of Aeromonas DNA hybridization group 11 in Aeromonas encheleia and extended descriptions of the species Aeromonas eucrenophila and A. encheleia . Int J Syst Bacteriol 47:1157–1164 [CrossRef]
    [Google Scholar]
  11. Huys G., Kämpfer P., Albert M. J., Kühn I., Denys R., Swings J. 2002; Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980). Int J Syst Evol Microbiol 52:705–712 [CrossRef]
    [Google Scholar]
  12. Huys G., Pearson M., Kämpfer P., Denys R., Cnockaert M., Inglis V., Swings J. 2003; Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand. Int J Syst Evol Microbiol 53:885–891 [CrossRef]
    [Google Scholar]
  13. Huys G., Cnockaert M., Swings J. 2005; Aeromonas culicicola Pidiyar et al. 2002 is a later subjective synonym of Aeromonas veronii Hickman-Brenner et al. 1987. Syst Appl Microbiol 28:604–609 [CrossRef]
    [Google Scholar]
  14. Kämpfer P., Christmann C., Swings J., Huys G. 1999; In vitro susceptibilities of Aeromonas genomic species to 69 antimicrobial agents. Syst Appl Microbiol 22:662–669 [CrossRef]
    [Google Scholar]
  15. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetic analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  16. Martin-Carnahan A., Joseph S. W. 2005; Genus I. Aeromonas Stanier 1943, 213AL . In Bergey's Manual of Systematic Bacteriology vol. 2, part B p  557–578 Edited by Garrity G. M., Brenner D. J., Krieg N. R., Staley J. T. New York: Springer;
    [Google Scholar]
  17. Martínez-Murcia A. J., Antón A. I., Rodríguez-Valera F. 1999; Patterns of sequence variation in two regions of the 16S rRNA multigene family of Escherichia coli . Int J Syst Bacteriol 49:601–610 [CrossRef]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  19. Miñana-Galbis D., Farfán M., Lorén J. G., Fusté M. C. 2002; Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from environmental and clinical samples in Spain. J Appl Microbiol 93:420–430 [CrossRef]
    [Google Scholar]
  20. Miñana-Galbis D., Farfán M., Fusté M. C., Lorén J. G. 2004; Aeromonas molluscorum sp. nov., isolated from bivalve molluscs. Int J Syst Evol Microbiol 54:2073–2078 [CrossRef]
    [Google Scholar]
  21. Morandi A., Zhaxybayeva O., Gogarten J. P., Graf J. 2005; Evolutionary and diagnostic implications of intragenomic heterogeneity in the 16S rRNA gene in Aeromonas strains. J Bacteriol 187:6561–6564 [CrossRef]
    [Google Scholar]
  22. Pavan M. E., Abbott S. L., Zorzópulos J., Janda J. M. 2000; Aeromonas salmonicida subsp. pectinolytica subsp. nov., a new pectinase-positive subspecies isolated from a heavily polluted river. Int J Syst Evol Microbiol 50:1119–1124 [CrossRef]
    [Google Scholar]
  23. Pidiyar V., Kaznowski A., Narayan N. B., Patole M., Shouche Y. S. 2002; Aeromonas culicicola sp. nov., from the midgut of Culex quinquefasciatus . Int J Syst Evol Microbiol 52:1723–1728 [CrossRef]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Soler L., Yáñez M. A., Chacon M. R., Aguilera-Arreola M. G., Catalán V., Figueras M. J., Martínez-Murcia A. J. 2004; Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes. Int J Syst Evol Microbiol 54:1511–1519 [CrossRef]
    [Google Scholar]
  26. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  27. Valera L., Esteve C. 2002; Phenotypic study by numerical taxonomy of strains belonging to the genus Aeromonas . J Appl Microbiol 93:77–95 [CrossRef]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  29. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2.4.1–2.4.5 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A, Struhl K. New York: Greene Publishing and Wiley-Interscience;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64497-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64497-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error