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Volume 58, Issue 10

sp. nov., isolated from the Pindari glacier of the Indian Himalayas Free

Abstract

Strain PN5 is a Gram-positive, aerobic, motile, rod-shaped, peritrichously flagellated bacterium that was isolated from the Pindari glacier using nutrient agar medium. Cells of PN5 are catalase-positive and oxidase-negative and contain lysine, glutamic acid and alanine in the peptidoglycan (peptidoglycan type A4). Further, the cells are characterized by the presence of iso-C and iso-C as the predominant fatty acids and MK-7 as the isoprenoid quinone. Based on the above characteristics, strain PN5 was assigned to the genus . Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain PN5 clustered with the type strain of with a sequence similarity of 97.2 %. DNA–DNA hybridization between PN5 and DSM 12223 resulted in a relatedness of only 15 %, clearly indicating that strain PN5 represents a novel species. Further, PN5 was different from with respect to various phenotypic and chemotaxonomic characteristics. Therefore, strain PN5 is identified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. is unique among psychrotolerant species in containing -Lys–-Glu in the cell-wall peptidoglycan. The type strain is PN5 (=LMG 23935 =MTCC9127 =JCM 15113).

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Keyword(s): Dap, diaminopimelic acid
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2008-10-01
2024-04-19
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Bacillus cecembensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas
Int J Syst Evol Microbiol 58, 2330 (2008); https://doi.org/10.1099/ijs.0.65515-0
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Transmission electron photomicrograph of a negatively stained cell of sp. nov. PN5 . Bar, 0.5 µm.

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Phylogenetic trees based on 16S rRNA gene sequences (1478 bases) reconstructed using UPGMA (a), maximum evolution (b) and DNAPARS (c), showing the phylogenetic relationships between psychrotolerant species of . [PDF](23 KB)

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