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Volume 65, Issue Pt_12

sp. nov., a spherical spirochaete isolated from cultures of JL01 Free

Abstract

An anaerobic, saccharolytic bacterial strain designated GLS2 was isolated from aggregates of the psychrotolerant archaeon strain JL01 isolated from arctic permafrost. Bacterial cells were non-motile, spherical, ovoid and annular with diameter 0.2–4 μm. They were chemoorganoheterotrophs using a wide range of mono-, di- and trisaccharides as carbon and energy sources. The novel isolate required yeast extract and vitamins for growth. The bacteria exhibited resistance to a number of β-lactam antibiotics, rifampicin, streptomycin and vancomycin. Optimum growth was observed between 30 and 34 °C, at pH 6.8–7.5 and with 1–2 g NaCl l. Isolate GLS2 was a strict anaerobe but it tolerated oxygen exposure. On the basis of 16S rRNA gene sequence similarity, strain GLS2 was shown to belong to the genus within the family . Its closest relatives were Buddy (99.3 % 16S rRNA gene sequence similarity) and Grapes (95.4 % similarity). The G+C content of DNA was 47.2 mol%. The level of DNA–DNA hybridization between strains GLS2 and Buddy was 34.7 ± 8.8 %. Major polar lipids were phosphoglycolipids, phospholipids and glycolipids; major fatty acids were C, C, C 3-OH, C dimethyl acetal (DMA), C and C DMA; respiratory quinones were not detected. The results of DNA–DNA hybridization, physiological and biochemical tests demonstrated genotypic and phenotypic differentiation of strain GLS2 from the four species of the genus with validly published names that allowed its separation into a new lineage at the species level. Strain GLS2 therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain GLS2 ( = DSM 26261 = VKM B-2742).

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Funding
This study was supported by the:
  • Russian Foundation of Basic Research (Award 15-04-08612)
  • President of Russia (Award MK-4530.2015.4)
© 2015 IUMS
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2015-12-01
2024-04-24
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Sphaerochaeta associata sp. nov., a spherical spirochaete isolated from cultures of Methanosarcina mazei JL01
Int J Syst Evol Microbiol 65, 4315 (2015); https://doi.org/10.1099/ijsem.0.000575
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