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Volume 65, Issue Pt_12

sp. nov., a moderately halophilic bacterium isolated from a saltern Free

Abstract

A novel pink-pigmented bacterial strain, UAH-SP71, was isolated from a saltern located in Santa Pola, Alicante (Spain) and the complete genome sequence was analysed and compared with that of M19-40, suggesting that the two strains constituted two separate species, with a 77.3 % ANI value. In this paper, strain UAH-SP71 was investigated in a taxonomic study using a polyphasic approach. Strain UAH-SP71 was a Gram-stain-negative, strictly aerobic, non-motile curved rod that grew in media containing 5–20 % (w/v) NaCl (optimum 10 % NaCl), at 5–40 °C (optimum 37 °C) and at pH 5–10 (optimum pH 8). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain UAH-SP71 is a member of the genus , showing a sequence similarity of 96.5 % with M19-40. Other related species are also members of the family , including RS91 (95.5 % 16S rRNA gene sequence similarity), ATCC 49307 (95.4 %) and MLHE-1 (94.9 %). DNA–DNA hybridization between strain UAH-SP71 and M19-40 was 39 %. The major cellular fatty acids of strain UAH-SP71 were Cω6 and/or Cω7, C, Cω6 and/or Cω7, C 3-OH and C, a pattern similar to that of M19-40. Phylogenetic, phenotypic and genotypic differences between strain UAH-SP71 and M19-40 indicate that strain UAH-SP71 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is UAH-SP71 ( = CECT 8396 = DSM 28542).

  • Published Online:
Funding
This study was supported by the:
  • Spanish Ministry of Economy and Competitiveness
© 2015 IUMS
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2015-12-01
2024-04-19
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Spiribacter curvatus sp. nov., a moderately halophilic bacterium isolated from a saltern
Int J Syst Evol Microbiol 65, 4638 (2015); https://doi.org/10.1099/ijsem.0.000621
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