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Volume 67, Issue 4

gen. nov., sp. nov., isolated from grass-field soil Free

Abstract

A novel aerobic, Gram-stain-negative and non-motile bacterial strain, designated ED7, was isolated from grass-field soil in Cheonan, Korea. Strain ED7 utilized methanol and methylamine, but not formate, as carbon and energy sources. The strain was able to grow at 20–42 °C (optimum 30–35 °C), at pH 7.0–8.5 (optimum pH 7.5–8.0), and in the absence of NaCl. According to the similarities of the 16S rRNA gene sequences, strain ED7 was most closely related to the genera (≤93.3 % 16S rRNA gene sequence similarity), (≤93.1 %) and (≤93.1 %). A phylogenetic analysis based on the 16S rRNA gene sequence of strain ED7 revealed that it was affiliated with the family , being most closely related to the genus In contrast to and , strain ED7 did not contain the gene. The DNA G+C content of the genomic DNA of strain ED7 was 71.8 mol%. The predominant fatty acids of strain ED7 were summed feature 8 (Cω7 and/or Cω6), summed feature 2 (C 3-OH, and/or C iso I), 11-methyl Cω7 and C 3-OH. The major isoprenoid quinone was ubiquinone 10 (Q-10). The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminophospholipid. Based on phenotypic, chemotaxonomic and genotypic characteristic data, strain ED7 could be differentiated from other genera, suggesting that strain ED7 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is ED7 (=JCM 30603=KCTC 42450).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Alphaproteobacterium , Chthonobacter albigriseus , ED7 , grass-field soil and Methylocystaceae
© 2017 IUMS
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2017-04-01
2024-04-18
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Chthonobacter albigriseus gen. nov., sp. nov., isolated from grass-field soil
Int J Syst Evol Microbiol 67, 883 (2017); https://doi.org/10.1099/ijsem.0.001695
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