1887

Abstract

A novel psychrotolerant bacterium, strain ISLP-3, was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram-positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase-positive. Growth occurred at 3–35 °C with an optimum at 22–24 °C, 0–2 % (w/v) NaCl with an optimum at 0.3 % and pH 6.2–9.5 with an optimum at pH 7.5. Strain ISLP-3 grew on several carbon sources, with the best growth on cellobiose. The isolate possessed ureolytic activity but growth was inhibited by urea. The strain was sensitive to: ampicillin, gentamycin, kanamycin rifampicin, tetracycline and chloramphenicol. Major fatty acids were: anteiso-C, iso-C, C, C and iso-C. The predominant menaquinone was MK-9(H). The genomic G+C content was 69.5 mol%. The 16S rRNA gene showed 99 % sequence similarity to that of ST-26, but their genes shared ≤91 % sequence similarity, suggesting that this new isolate represents a novel species within the genus . This conclusion was supported by average nucleotide identity, which was ≤91 % to the most closely related strain. The name sp. nov. is proposed for the novel species with the type strain ISLP-3=ATCC TSD-17=DSM 100501=JCM 30887). The complete genome draft sequence of ISLP-3 was deposited under IMG OID 2657245272. Emendments to the descriptions of related taxa have been made based on experimental data from our comparative analysis.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001838
2017-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/5/1442.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001838&mimeType=html&fmt=ahah

References

  1. Fernández-Garayzábal JF, Dominguez L, Pascual C, Jones D, Collins MD. Phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of Sanguibacter gen.nov. Lett Appl Microbiol 1995; 20:69–75 [View Article][PubMed]
    [Google Scholar]
  2. Huang Y, Dai X, He L, Wang YN, Wang BJ et al. Sanguibacter marinus sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2005; 55:1755–1758 [View Article][PubMed]
    [Google Scholar]
  3. Hong SG, Lee YK, Yim JH, Chun J, Lee HK. Sanguibacter antarcticus sp. nov., isolated from antarctic sea sand. Int J Syst Evol Microbiol 2008; 58:50–52 [View Article][PubMed]
    [Google Scholar]
  4. Kim MK, Pulla RK, Kim SY, Yi TH, Soung NK et al. Sanguibacter soli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2008; 58:538–541 [View Article][PubMed]
    [Google Scholar]
  5. Pascual C, Collins MD, Grimont PAD, Domínguez L, Fernández-Garayzábal JF. Sanguibacter inulinus sp. nov. Int J Syst Evol Microbiol 1996; 46:811–813
    [Google Scholar]
  6. Hoover RB. 2008; Schirmacher oasis/Lake untersee Antarctica astrobiology Expe-ditions. Explorer's Club Expedition Report Flag #162. https://explorers.org/flag_reports/Flag_162_Report_-_Richard_B._Hoover_9-29-08_.pdf
  7. Guisler MD, Pikuta EV, Townsend AM, Hoover RB. Psychrotolerant anaerobes from Lake Podprudnoye, Antarctica and penguin Spheniscus demersus colony, South Africa. In Retherford KD, Hoover RB, Levin GV, YuRozanov A. (editors) Instruments and Methods for Astrobiology and Planetary Missions XII vol. 7441 SPIE; 2009 pp. 74410L-1-12
    [Google Scholar]
  8. Townsend A, Pikuta EV, Guisler M, Stahl S, Hoover RB et al. Anaerobic psychrophiles from Lake Zub and Lake Untersee, Antarctica. In Retherford KD, Hoover RB, Levin GV, YuRozanov A. (editors) Instruments and Methods for Astrobiology and Planetary Missions XII vol. 7441 SPIE; 2009 pp. 74410K-1-12
    [Google Scholar]
  9. Hoover RB, Pikuta EV. Psychrophilic and psychrotolerant microbial extremophiles in polar environments. In Bej AK, Aislabie J, Atlas RM. (editors) Polar Microbiology: The Ecology, Biodiversity and Bioremediation Potential of Microorganisms in Extremely Cold Environments Atlas: CRC Press; 2009 pp. 115–156 [CrossRef]
    [Google Scholar]
  10. Wolin EA, Wolin MJ, Wolfe RS. Formation of methane by bacterial extracts. J Biol Chem 1963; 238:2882–2886[PubMed]
    [Google Scholar]
  11. Whitman WB, Ankwanda E, Wolfe RS. Nutrition and carbon metabolism of Methanococcus voltae. J Bacteriol 1982; 149:852–863[PubMed]
    [Google Scholar]
  12. Murray RGE, Doetsch RN, Robinow CF. Determinative and cytological light microscopy. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 21–41
    [Google Scholar]
  13. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  14. Pikuta EV, Menes RJ, Bruce AM, Lyu Z, Patel NB et al. Raineyella antarctica gen. nov., sp. nov., a novel psychrotolerant, D-amino acid utilizing anaerobe isolated from two geographic locations of the southern hemisphere. Int J Syst Evol Microbiol 2016; 66:5529–5536 [View Article][PubMed]
    [Google Scholar]
  15. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996; 42:989–1005 [View Article]
    [Google Scholar]
  16. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988; 38:358–361 [View Article]
    [Google Scholar]
  17. Miller LT. A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J Clin Microbiol 1982; 16:584–586
    [Google Scholar]
  18. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
    [Google Scholar]
  19. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202 [CrossRef]
    [Google Scholar]
  20. Altenburger P, Kämpferb P, Makristathisc A, Lubitza W, Bussea H-J. Classification of bacteria isolated from a medieval wall painting. J Biotechnol 1996; 47:39–52 [View Article]
    [Google Scholar]
  21. Stolz A, Busse HJ, Kämpfer P. Pseudomonas Knackmussii sp. nov. Int J Syst Evol Microbiol 2007; 57:572–576 [View Article][PubMed]
    [Google Scholar]
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  23. Logan NA, De Vos P. Genus I. Bacillus Cohn 1872, 174AL. In De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W et al. (editors) Bergey’s Manual of Systematics of Archaea and Bacteria, 2nd ed. vol. 3 New York: Springer; 2009 pp. 21–128
    [Google Scholar]
  24. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002; 52:1043–1047 [View Article][PubMed]
    [Google Scholar]
  25. Holmes DE, Nevin KP, Lovley DR. Comparison of 16S rRNA, nifD, recA, gyrB, rpoB and fusA genes within the family Geobacteraceae fam. nov. Int J Syst Evol Microbiol 2004; 54:1591–1599 [View Article][PubMed]
    [Google Scholar]
  26. Yong T, Hong J, Zhangfu L, Li Z, Xiuqiong D et al. Purification and characterization of an extracellular chitinase produced by bacterium C4. Ann Microbiol 2005; 55:213–218
    [Google Scholar]
  27. Park HJ, Kim D, Kim IH, Lee C-E, Kim I-C et al. Characteristics of cold-adaptive endochitinase from antarctic bacterium Sanguibacter antarcticus KOPRI 21702. Enzyme Microb Technol 2009; 45:391–396 [View Article]
    [Google Scholar]
  28. Stackebrandt E, Schumann P. Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder micrococcineae. Int J Syst Evol Microbiol 2000; 50:1279–1285 [View Article][PubMed]
    [Google Scholar]
  29. Pascual CR, Fernández-Garayzábal JF. Family XV. Sanguibacteraceae Stackebrandt and Schumann 2000, 1284VP. In Goodfellow M, Kämpfer P, Busse H-J, Trujillo ME, Suzuki K et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 5 The Actinobacteria, Part A 2012 pp. 1027–1034
    [Google Scholar]
  30. Buchanan RE. Studies in the nomenclature and classification of the bacteria: II. the primary subdivisions of the schizomycetes. J Bacteriol 1917; 2:155–164[PubMed]
    [Google Scholar]
  31. Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a new hierarchic classification system, actinobacteria classis nov. Int J Syst Bacteriol 1997; 47:479–491 [View Article]
    [Google Scholar]
  32. Varghese NJ, Mukherjee S, Ivanova N, Konstantinidis KT, Mavrommatis K et al. Microbial species delineation using whole genome sequences. Nucleic Acids Res 2015; 43:6761–6771 [View Article][PubMed]
    [Google Scholar]
  33. Konstantinidis KT, Tiedje JM. Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci USA 2005; 102:2567–2572 [View Article][PubMed]
    [Google Scholar]
  34. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci 2004; 101:11030–11035 [View Article]
    [Google Scholar]
  35. Brown JM, Frazier RP, Morey RE, Steigerwalt AG, Pellegrini GJ et al. Phenotypic and genetic characterization of clinical isolates of CDC coryneform group A-3: proposal of a new species of Cellulomonas, cellulomonas denverensis sp. nov. J Clin Microbiol 2005; 43:1732–1737 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001838
Loading
/content/journal/ijsem/10.1099/ijsem.0.001838
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error