1887

Abstract

A gram-negative, rod shaped bacterium designated as strain H2 was isolated from an artificial pond in Korea. The strain H2 was able to grow aerobically and anaerobically with optimal growth occurring at 30 °C and pH 7.0 under aerobic conditions. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain H2 belonged to the genus of the family . Phylogenetic similarity calculated from 16S rRNA gene sequences of strain H2 and valid species belongs to the genus ranged from 93.2 % (for cf) to 94.4 % (for LY03), and strain H2 formed a tight monophyletic group with them. Predominant fatty acids were C and summed feature 3, which consisted of C 6 and/or C 7. The major respiratory quinone of the strain H2 was ubiquinone-8, and DNA G+C content was 60.2 %. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, unidentified aminolipid, and unidentified phospholipid. The biochemical characteristics that distinguished strain H2 from other species included positive cystine arylamidase activity and lacked -chymotrypsin and -glucosidase (aesculin hydrolysis) activity. In addition, reciprocal DNA–DNA relatedness between H2 and three strains ranged from 32.0 to 43.7 %. On the basis of its phylogenetic, chemotaxonomic, and genotypic characteristics, strain H2 represents a novel species of the genus sp. nov. is proposed with the type strain H2 (=KCTC 52574=LMG 29894).

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/content/journal/ijsem/10.1099/ijsem.0.002195
2017-11-01
2024-03-28
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