1887

Abstract

A strictly anaerobic bacterial strain (SH021) was isolated from a methanogenic reactor. Cells were Gram-stain-positive, motile, straight or slightly curved rods. The optimum temperature for growth was 35 °C, and the optimum pH was 6.1–7.7. The strain was asaccharolytic and utilized amino acids as growth substrates. The strain produced acetate and propionate from -alanine and -serine, and propionate and butyrate from -threonine. Branched-chain amino acids (-isoleucine, -leucine and -valine) were utilized weakly, and isovalerate or isobutyrate was produced. Strain SH021 utilized pyruvate and lactate, and converted them to acetate and propionate. The genomic DNA G+C content was 38.2 mol%. Compounds related to iso-C were detected as major components in the cellular fatty acids analysis. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. On the basis of 16S rRNA gene sequences, the most closely related known species were , and in cluster XIVb of the class . Based on the phylogenetic and phenotypic data, gen. nov., sp. nov. is proposed to accommodate strain SH021 (=JCM 31556=DSM 103575). For the three related species of the genus , comb. nov. (type strain DSM 1682=JCM 1430=ATCC 25522=CCUG 9280=NCIMB 10656=VPI 5303), comb. nov. (type strain X4=DSM 3847=KCTC 15564) and comb. nov. (type strain G17=DSM 14214=LMG 20954) are proposed with emended descriptions of these species.

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2017-10-01
2024-03-29
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