1887

Abstract

Strain PFL01 was isolated from traditional Korean fermented clam, jogae-jeotgal, and characterized. The strain was a facultative anaerobic, Gram-stain-negative bacterium that was rod-shaped, motile and beige-pigmented. The phylogenetic sequence analysis based on the 16S rRNA gene from PFL01 revealed that it was closely related to LMG 10245 and LMG 2784 with 99.3 and 99.3 % sequence identities, respectively. Multilocus sequence type analysis of concatenated partial , , and gene sequences showed a clear distinction of strain PFL01 from its closest related type strains. The discrimination was also supported by unique repetitive extragenic palindromic PCR (Rep-PCR, ERIC-PCR) fingerprint patterns. In addition, results from average nucleotide identity analyses with other species were less than 85 %. and API analyses revealed distinct characteristics from other species of . The cellular fatty acid profile of the strain consisted of C, cyclo-C, Cω7/Cω6 and Cω7/Cω6 as major components. The whole genome of PFL01 was 4.6 Mb with a G+C content of 55.3 mol%. Based on these results, strain PFL01 was classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain in PFL01 (=KCCM 43247=JCM 31901).

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2018-05-01
2024-03-29
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