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Volume 70, Issue 3

gen. nov., sp. nov., belonging to the , isolated from a temperate freshwater habitat Free

Abstract

The bacterial strain 53C-WASEF was isolated from a small freshwater ditch located in Eugendorf, Austria. Phylogenetic reconstructions with 16S rRNA gene sequences and genome based, with amino acid sequences obtained from 105 single copy genes, suggested that the strain represents a new genus and a new species within the family , which belongs to the class of the phylum . Comparisons of the 16S rRNA gene sequence of strain 53C-WASEF with those of related type strains revealed a highest sequence similarity of 93.5 % to and of 92.9 % to . Interestingly, phylogentic trees indicated the latter as being the closest known relative of the new strain. Phenotypic, chemotaxonomic and genomic traits were investigated. Cells were observed to be small, spherical, motile and unpigmented, and grew chemoorganotrophically and aerobically. The respiratory quinone was MK-7, the predominant fatty acids were anteiso-C, Cω5 and C. The identified polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Genome sequencing revealed genes putatively encoding for flagella synthesis and cellulose degradation. The genome size was 4.1 Mbp and the G+C content 60.6 mol%. For the new genus and the new species, we propose the name gen. nov., sp. nov. (=CIP 111665=DSM 109123).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): freshwater , Opitutaceae , Rariglobus and Verrucomicrobia
© 2020 The Authors
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2020-01-20
2024-05-07
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Rariglobus hedericola gen. nov., sp. nov., belonging to the Verrucomicrobia, isolated from a temperate freshwater habitat
Int J Syst Evol Microbiol 70, 1830 (2020); https://doi.org/10.1099/ijsem.0.003980
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