1887

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Volume 70, Issue 3

gen. nov., sp. nov. and sp. nov., two new members of the family isolated from the rhizosphere of Free

Abstract

Two strains of the family were isolated from the rhizosphere of the medicinal plant . Cells of both strains were Gram-stain-negative, motile by means of a single polar flagellum, non-spore-forming, non-capsulated, short rods that divided by binary fission. Colonies were small and white. Strains R5913 and R5959 were oxidase-positive, mesophilic, neutrophilic and grew optimally without NaCl. Both grew under aerobic and microaerophilic conditions and on a limited range of substrates with best results on yeast extract. Major fatty acids were C cyclo 8 and C; in addition, Cω7 was also found as a predominant fatty acid in strain R5913. The major respiratory quinone was ubiquinone 10 (Q-10). The DNA G+C contents of strains R5913 and R5959 were 66.0 and 67.4 mol%, respectively. 16S rRNA gene sequence comparison revealed that the closest relatives (<92 % similarity) of the strains are MCCC 1A02656, CGMCC 1.7660, D78 and 04SU4-P. The two novel strains shared 98.6 % sequence similarity and represent different species on the basis of low average nucleotide identity of their genomes (83.8 %). Based on the combined phenotypic, genomic and phylogenetic investigations, the two strains represent two novel species of a new genus in the family , for which the name gen. nov. is proposed, comprising the type species sp. nov. (type strain R5913=DSM 109816=CECT 9472) and sp. nov. (type strain R5959=DSM 109817=CECT 9620).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Hypericum perforatum , rhizosphere and Rhodospirillaceae
Funding
This study was supported by the:
  • DAAD (Award 91621463)
    • Principle Award Recipient: Zahra Noviana
© 2020 The Authors
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2020-01-20
2024-04-24
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Hypericibacter terrae gen. nov., sp. nov. and Hypericibacter adhaerens sp. nov., two new members of the family Rhodospirillaceae isolated from the rhizosphere of Hypericum perforatum
Int J Syst Evol Microbiol 70, 1850 (2020); https://doi.org/10.1099/ijsem.0.003983
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