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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

sp. nov., a novel arsenic-resistant bacterium Free

Abstract

A lactic acid bacterial strain, HBUAS57009, isolated from traditionally fermented food (Zha-Chili) in China, was characterized to clarify its taxonomic status using a polyphasic approach. Strain HBUAS57009 was phylogenetically closely related to DCY50, 218-6, 112-3, TUM BP 140423000-2250, 218-10 and THK-V8 with sequence similarities of 98.6–99.3 %. The genome-to-genome distance and average nucleotide identity values between the genomes of strain HBUAS57009 and type strains of closely related species were less than 32.0 and 86.0 %, respectively; this is below the threshold for species boundaries. The major cellular fatty acids (>10 %) were C, C ω9 and iso-C. The G+C content of the genomic DNA of strain HBUAS57009 was 47.8 mol%. Examination of the functional categories of the genome revealed that strain HBUAS57009 could perform both homolactic and heterolactic fermentation processes to produce lactic acid via complete glycolysis and the pentose phosphate pathway. The putative biosynthesis pathway of butane-2,3-diol and acetoin, two important flavour compounds in the food industry, were identified using mapper analysis. Based on its genotypic and phenotypic features, strain HBUAS57009 (=GDMCC 1.1664=KACC 21424) is designated as the type strain of a novel species, for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Flavour compound , Genome , Lactic acid bacteria and Phylogeny
Funding
This study was supported by the:
  • Projects of Innovation and Development Pillar Program for Key Industries in Southern Xinjiang of Xinjiang Production and Construction Corps (Award 2018DB002)
    • Principle Award Recipient: Zhuang Guo
  • Xinjiang Production and Construction Corps Innovation Team Building Projects in Key Areas (Award 2017CB012)
    • Principle Award Recipient: Zhuang Guo
  • Cultivation of Teachers' Scientific Research Ability Foundation of Hubei University of Arts and Science (Award 2017kypy051)
    • Principle Award Recipient: Zhuang Guo
  • National Natural Science Foundation of China (Award 31600947)
    • Principle Award Recipient: Zhuang Guo
  • National Natural Science Foundation of China (Award 31501455)
    • Principle Award Recipient: Zhuang Guo
© 2020 The Authors
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2020-03-04
2024-04-18
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Lactobacillus enshiensis sp. nov., a novel arsenic-resistant bacterium
Int J Syst Evol Microbiol 70, 2580 (2020); https://doi.org/10.1099/ijsem.0.004072
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