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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 9

sp. nov., a bacterium isolated from sediment collected at a cold seep field in the South China Sea Free

Abstract

A novel bacterium, designated strain KXZD1103, was isolated from sediment collected at a cold seep field of the Formosa Ridge in the South China Sea. Cells were Gram-stain-negative, facultatively anaerobic, motile, oxidase- and catalase-positive, and grew optimally at 28 °C, pH 6.0–pH 7.0 and in the presence of 1–3 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C ω7/C ω6), summed feature 3 (C ω7/C ω6) and C. The major respiratory ubiquinone was Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Analysis of 16S rRNA gene sequences revealed that strain KXZD1103 grouped with members of the genus , with 4CA (98.1 % sequence similarity) and R4-8 (97.7 %) as its closest neighbours. Genome sequencing revealed a genome size of 4.17 Mb and a DNA G+C content of 50.1 %. Genomic average nucleotide identity values for strain KXZD1103 with the type strains within the genus ranged from 71.0 to 75.7 %, while the DNA–DNA hybridization values for strain KXZD1103 with these strains ranged from 16.1 to 21.6 %. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses, strain KXZD1103 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KXZD1103 (=KCTC 72678=MCCC 1K04283).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): chemotaxonomic characterization , cold seep , deep sea , genome , Nitrincola and type strain
Funding
This study was supported by the:
  • Senior User Project of RV KEXUE (Award KEXUE2019G06)
    • Principle Award Recipient: Huan Zhang
  • Open Research Project of National Major Science & Technology Infrastructure (RV KEXUE) (Award NMSTI-KEXUE2017K01)
    • Principle Award Recipient: Chaolun Li
  • National Key R&D Program of China (Award 2018YFC0310800)
    • Principle Award Recipient: Huan Zhang
© 2020 The Authors
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2020-07-31
2024-05-09
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Nitrincola iocasae sp. nov., a bacterium isolated from sediment collected at a cold seep field in the South China Sea
Int J Syst Evol Microbiol 70, 4897 (2020); https://doi.org/10.1099/ijsem.0.004352
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