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Volume 28, Issue 3

, a New Genus of Nonfruiting, Gliding Bacteria with a High Base Ratio Free

Abstract

Highly mucoid, cream, pink and yellow-brown gliding organisms having deoxyribonucleic acid guanine-plus-cytosine contents of 62 to 70.1 mol% have been isolated by several workers, but since these organisms have never been observed to produce typical myxobacterial fruiting bodies, their taxonomy has been problematical. Forty-six isolates were studied in detail, among them Ensign and Wolfe’s organism AL-1 and Cook’s isolate 495, both of which produce important proteases, as well as Cook’s culture 3C, which elaborates the potent, wide-spectrum antibiotic myxin. A new genus, , has been established for these organisms, and four new species and one new subspecies have been named and described: (type strain, ATCC 29479), (type strain, ATCC 29482), (type strain, ATCC 29487), subspecies Christensen (type strain, ATCC 29488), and (type strain, ATCC 29489). The dimensions of the thin, gliding, flexing cells of are 0.3 to 0.5 by 1.0 to 15.0 (sometimes up to 70) μm. These soil and water organisms all degrade chitin, two degrade alginate, three degrade pectate, three degrade carboxymethylcellulose, and one degrades starch, but none decomposes filter paper or agar. They are strongly proteolytic and characteristically lyse a variety of microorganisms such as gram-negative, gram-positive (including actinomycetes), and blue-green bacteria, fungi, and green algae, as well as nematodes. The genus has been placed in a new family, , within a new order,

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1978-07-01
2024-04-23
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References

  1. Allen L. C., Whitaker D. R. 1972; The /3-lytic protease of myxobacter 495: aspects of its specificity. Proc. Can. Fed. Biol. Soc 15:#31
     [Google Scholar]
  2. American Type Culture Collection 1974 Catalogue of strains (bacteria), 11th. American Type Culture Collection; Rockville, Md:
     [Google Scholar]
  3. Bailey W. R., Scott E. G. 1970 Diagnostic microbiology. , 3rd. Mosby; St. Louis, Mo:
     [Google Scholar]
  4. Behki R. M., Lesley S. M. 1972; Deoxyribonucleic acid degradation and the lethal effect by myxin in Escherichia coli. J. Bacteriol. 109:250–261
     [Google Scholar]
  5. Buchanan R. E., Gibbons N. E.ed 1974 Bergey’s manual of determinative bacteriology. Williams and Wilkins Co.; Baltimore:
     [Google Scholar]
  6. Christensen P. J. 1974; A new approach to the description of colony colour of cytophagas and their allies. Can. J. Microbiol. 20:937–942
     [Google Scholar]
  7. Christensen P. J. 1977; Synonymy of Flavobacterium pectinovorum Dorey with Cytophaga johnsonae Stan- ier. Int. J. Syst. Bacteriol. 27:122–132
     [Google Scholar]
  8. Christensen P. J., Cook F. D. 1972; The isolation and enumeration of cytophagas. Can. J. Microbiol. 18:1933–1940
     [Google Scholar]
  9. Daft M. J., Stewart W. D. P. 1971; Bacterial pathogens of fresh-water blue-green algae. New Phytol. 70:819–829
     [Google Scholar]
  10. Damoglou A. P., Allen L. C., Roy C., Whitaker D. R. 1972; The /3-lytic protease of myxobacter 495: aspects of its structure. Proc. Can. Fed. Biol. Soc 15:#27
     [Google Scholar]
  11. Dworkin M., Gibson S. M. 1964; A system for studying microbial morphogenesis: rapid formation of microcysts in Myxococcus xanthus. Science 146:243–244
     [Google Scholar]
  12. Ensign J. C., Wolfe R. S. 1965; Lysis of bacterial cell walls by an enzyme isolated from a myxobacter. J. Bacteriol. 90:395–402
     [Google Scholar]
  13. Ensign J. C., Wolfe R. S. 1966; Characterization of a small proteolytic enzyme which lyses bacterial cell walls. J. Bacteriol. 91:524–534
     [Google Scholar]
  14. Gillespie D. C., Cook F. D. 1965; Extracellular enzymes from strains of Sorangium. Can. J. Microbiol. 11:109–118
     [Google Scholar]
  15. Hedges A., Wolfe R. S. 1974; Extracellular enzyme from myxobacter AL-1 that exhibits both B-l, 4-glucan- ase and chitosanase activities. J. Bacteriol. 120:844–853
     [Google Scholar]
  16. Jackson R. L., Matsueda G. R. 1970; Myxobacter AL-1 protease. Methods Enzymol. 19:591–599
     [Google Scholar]
  17. Jackson R. L., Wolfe R. S. 1968; Composition, properties and substrate specificities of Myxobacter AL- 1 protease. J. Biol. Chem. 243:879–888
     [Google Scholar]
  18. Katznelson H., Gillespie D. C., Cook F. D. 1964; Studies on the relationships between nematodes and other soil micro-organisms. III. Lytic action of soil myxobacters on certain species of nematodes. Can. J. Microbiol. 10:699–704
     [Google Scholar]
  19. Lesley S. M., Behki R. M. 1967; Mode of action of myxin on E. coli. J. Bacteriol. 94:1837–1845
     [Google Scholar]
  20. Lesley S. M., Behki R. M. 1971; Recovery of metabolic activity in E. coli following limited exposure to myxin. Can. J. Microbiol. 17:1327–1333
     [Google Scholar]
  21. Lesley S. M., Behki R. M., Gillespie D. C. 1967; Production of radioactive myxin. Can. J. Microbiol. 13:1251–1257
     [Google Scholar]
  22. Marmur J., Doty P. 1962; Determination of the base composition of DNA from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
     [Google Scholar]
  23. Martin S. M., So V. 1969; Solubilization of autoclaved feathers and wool by myxobacteria. Can. J. Microbiol. 15:1393–1397
     [Google Scholar]
  24. Matheson A. T., Mikulik K. 1972; The influence of growth conditions and isolation procedures on the proteolytic breakdown of ribosomal proteins from Myxobacter 495. Can. J. Microbiol. 18:355–359
     [Google Scholar]
  25. Pate J. L., Johnson J. L., Ordal E. J. 1967; The fine structure of Chondrococcus columnaris. II. Structure and formation of rhapidosomes. J. Cell. Biol. 35:15–35
     [Google Scholar]
  26. Pate J. L., Ordal E. J. 1967; The fine structure of Chondrococcus columnaris. I. Structure and formation of mesosomes. J. Cell. Biol. 35:1–14
     [Google Scholar]
  27. Peterson E. A., Gillespie D. C., Cook F. D. 1966; A wide-spectrum antibiotic produced by a species of Sorangium. Can. J. Microbiol. 12:221–230
     [Google Scholar]
  28. Peterson E. A., Katznelson H., Cook F. D. 1965; The influence of chitin and myxobacters on numbers of actinomyctes in soil. Can. J. Microbiol. 2:595–596
     [Google Scholar]
  29. Sendecki W., Mikulik K., Matheson A. T. 1971; Some properties of ribosomes from Myxobacter 495. Can. J. Biochem. 49:1333–1339
     [Google Scholar]
  30. Shilo M. 1970; Lysis of blue-green algae by myxobacter. J. Bacteriol. 104:453–461
     [Google Scholar]
  31. Sierra G. 1957; A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substances. Antonie van Leeuwenhoek J. Microbiol. Serol. 23:15–22
     [Google Scholar]
  32. Smit M., Clark A. G. 1971; The observation of myxobacterial fruiting bodies. J. Appl. Bacteriol. 34:399–401
     [Google Scholar]
  33. Soriano S. 1945; El nuevo orden Flexibacteriales y la clasificación de los órdenes de las bacterias. Rev. Argent. Agron. 12:120–140
     [Google Scholar]
  34. Stanier R. Y. 1957 Order VIII. Myxobacterales Jahn 1915. 854–891 Breed R. S., Murray E. G. D., Smith N. R.ed Bergey’s manual of determinative bacteriology, 7th. The Williams and Wilkins Co.; Baltimore:
     [Google Scholar]
  35. Stewart J. R., Brown R. M. 1969; Cytophaga that kills or lyses algae. Science 164:1523–1524
     [Google Scholar]
  36. Stewart J. R., Brown R. M. 1971; Algicidal nonfruiting myxobacteria with high G+C ratios. Arch. Mik- robiol. 80:176–190
     [Google Scholar]
  37. Tan I., Hartmann W., Guntermann U., Hütter- mann A., Kühlwein H. 1974; Studies on the cell cycle of Myxobacter AL-1. I. Size fractionation of exponentially growing cells by zonal centrifugation. Arch. Mikrobiol. 100:389–396
     [Google Scholar]
  38. Whitaker D. R. 1965; Lytic enzymes of Sorangium sp.: isolation and enzymatic properties of the a- and ftproteases. Can. J. Biochem. 43:1935–1954
     [Google Scholar]
  39. Whitaker D. R. 1967; Simplified procedures for production and isolation of the bacteriolytic proteases of Sorangium sp.. Can. J. Biochem. 45:991–993
     [Google Scholar]
  40. Whitaker D. R. 1970; The a-lytic protease of a myxo- bacterium. Methods Enzymol. 19:599–613
     [Google Scholar]
  41. Whitaker D. R., Cook F. D., Gillespie D. C. 1965; Lytic enzymes of Sorangium sp. Some aspects of enzyme production in submerged culture. Can. J. Biochem. 43:1927–1933
     [Google Scholar]
  42. Whitaker D. R., Roy C., Tsai C. S., Jurásek L. 1965; Lytic enzymes of Sorangium sp. A comparison of the proteolytic properties of the a- and /B-lytic proteases. Can. J. Biochem. 43:1961–1970
     [Google Scholar]
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Lysobacter, a New Genus of Nonfruiting, Gliding Bacteria with a High Base Ratio
Int J Syst Evol Microbiol 28, 367 (1978); https://doi.org/10.1099/00207713-28-3-367
/content/journal/ijsem/10.1099/00207713-28-3-367
/content/journal/ijsem/10.1099/00207713-28-3-367
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