@article{mbs:/content/journal/ijsem/10.1099/00207713-31-2-184, author = "Dürre, P. and Andersch, W. and Andreesen, J. R.", title = "Isolation and Characterization of an Adenine-Utilizing, Anaerobic Sporeformer, Clostridium purinolyticum sp. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1981", volume = "31", number = "2", pages = "184-194", doi = "https://doi.org/10.1099/00207713-31-2-184", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-31-2-184", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Strains of anaerobic, sporeforming bacteria were isolated by using adenine as a carbon and energy source. Strain WA-1 could utilize all naturally occurring purines, as well as such products of purine degradation as glycine and some of its derivatives, as substrates. The products formed were acetate, formate, carbon dioxide, and ammonia. The organism depended strictly on the availability of selenium compounds for growth. Selenite and molybdate supplementation of the medium promoted the formation of active formate dehydrogenase and xanthine dehydrogenase. The molar growth yield on adenine or hypoxanthine was 10.0 g of dry weight per mol of substrate. The organism’s doubling time was 80 min when the strain was grown at its optimum temperature, 36°C. The organism had a guanine plus cytosine content of 29 mol% (by the thermal denaturation method). It shared similarities with Clostridium acidiurici and “C. cylindrosporum” (not on the Approved Lists of Bacterial Names), but it could be differentiated from these by deoxyribonucleic acid-deoxyribonucleic acid homology. Therefore, it is described as a new species, Clostridium purinolyticum. The ability of the type strain, WA-1 (DSM 1384), to grow on adenine and glycine was the most significant difference between it and the two above-mentioned species.", }