1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 32, Issue 1

sp. nov., a Saccharolytic Species from Sewage Sludge † Free

Abstract

A new species of isolated from a methanogenic cellulose-enrichment culture of sewage sludge is described. The colonies produced by these bacteria were white, circular, and convex with smooth margins. The cells were straight, spindle-shaped rods, 0.6 by 3.0 μm in size. They were gram negative and nonmotile, and they formed round, terminal spores. A wide variety of carbohydrates was fermented by this mesophilic anaerobe. The major fermentation products were acetic acid, hydrogen, carbon dioxide, and ethanol. The deoxyribonucleic acid base composition was 28 mol% guanine plus cytosine. The name is proposed for this new species on the basis of its broad saccharolytic activity. The type strain of . is WM1 (= NRC 2533).

  • Published Online:
Copyright © 1982 International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-32-1-132
1982-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/32/1/ijs-32-1-132.html?itemId=/content/journal/ijsem/10.1099/00207713-32-1-132&mimeType=html&fmt=ahah

References

  1. Holdeman L. V., Moore W. E. C. 1973; Anaerobe laboratory manual. , 2. Anaerobe Laboratory, Virginia Polytechnic and State University; Blacksburg:
     [Google Scholar]
  2. Hungate R. E. 1950; The anaerobic mesophilic cellulolyt-ic bacteria. Bacteriol. Rev 14:1–49
     [Google Scholar]
  3. Johnson J. L., Francis B. S. 1975; Taxonomy of the clostridia: ribosomal ribonucleic acid homologies among the species. J. Gen. Microbiol 88:229–244
     [Google Scholar]
  4. Khan A. W. 1977; Anaerobic degradation of cellulose by element culture. Can. J. Microbiol 23:1700–1705
     [Google Scholar]
  5. Khan A. W., Trottier T. M. 1978; Effect of sulfur-containing compounds on anaerobic degradation of cellulose to methane by element cultures obtained from sewage sludge. Appl. Environ. Microbiol 35:1027–1034
     [Google Scholar]
  6. Khan A. W., Trottier T. M., Patel G. B., Martin S. M. 1979; Nutrient requirement for the degradation of cellulose to methane by a element population of anaerobes. J. Gen. Microbiol 112:365–372
     [Google Scholar]
  7. Markov G. C., Ivanov I. G. 1974; Hydroxyapatite column chromatography in procedures for isolation of purified DNA. Anal. Biochem 59:555–563
     [Google Scholar]
  8. Ohwaki K., Hungate R. E. 1977; Hydrogen utilization by clostridia in sewage sludge. Appl. Environ. Microbiol 33:1270–1274
     [Google Scholar]
  9. Patel G. B., Roth L. A. 1978; Acetic acid and hydrogen metabolism during coculture of an acetic acid producing bacterium with methanogenic bacteria. Can. J. Microbiol 24:1007–1010
     [Google Scholar]
  10. Smith L. D. S., Hobbs G. 1974; Clostridium. 551–572 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology The Williams & Wilkins Co.; Baltimore:
     [Google Scholar]
  11. Ulitzer S. 1972; Rapid determination of DNA base composition by ultraviolet spectroscopy. Biochim. Biophys. Acta 272:1–11
     [Google Scholar]
  12. Wieringa K. T. 1940; The formation of acetic acid from carbon dioxide and hydrogen by anaerobic sporeforming bacteria. Antonie van Leeuwenhoek J. Microbiol. Serol 6:251–262
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-32-1-132
Loading
Clostridium saccharolyticum sp. nov., a Saccharolytic Species from Sewage Sludge†
Int J Syst Evol Microbiol 32, 132 (1982); https://doi.org/10.1099/00207713-32-1-132
/content/journal/ijsem/10.1099/00207713-32-1-132
/content/journal/ijsem/10.1099/00207713-32-1-132
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error