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Volume 33, Issue 2

Taxonomy of the Determined by Using Immunoelectrophoresis Free

Abstract

The similarities of various strains of spp. and spp. to reference strains of , and were determined by rocket line immunoelectrophoresis. The strains of and used were immunologically more homogeneous than the strains of studied, possibly due to the more diverse geographical origins of the strains. Low values were obtained for the mean immunological distances (1 – proportion of immunoprecipitation bands shared between strains) between and strains, suggesting that these two species are immunologically closely related. Immunological distances from the reference strain were similar for and for other undisputed members of the genus , which makes it reasonable to retain in this genus. When the three antisera were used, all species had mean immunological distances of less than 0.5, whereas the species were immunologically more distant, showing that the six species of form an immunologically related group which is distinct from the species. Our results with the three antisera show that each species of is immunologically distant from the other species, as well as from the species. We compare our immunoelectrophoretic results with the molecular biological results of De Smedt et al. (Int. J. Syst. Bacteriol. :106–122, 1980) and the numerical taxonomic analysis of Thompson and Skerman (, Academic Press, Inc., London, 1979).

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Copyright © 1983 International Union of Microbiological Societies
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1983-04-01
2024-04-19
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Taxonomy of the Azotobacteraceae Determined by Using Immunoelectrophoresis
Int J Syst Evol Microbiol 33, 147 (1983); https://doi.org/10.1099/00207713-33-2-147
/content/journal/ijsem/10.1099/00207713-33-2-147
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