RT Journal Article SR Electronic(1) A1 KRUMHOLZ, L. R. A1 BRYANT, M. P.YR 1985 T1 Clostridium pfennigii sp. nov. Uses Methoxyl Groups of Monobenzenoids and Produces Butyrate JF International Journal of Systematic and Evolutionary Microbiology, VO 35 IS 4 SP 454 OP 456 DO https://doi.org/10.1099/00207713-35-4-454 PB Microbiology Society, SN 1466-5034, AB The new bacterial species Clostridium pfennigii obtained energy for growth by catabolizing pyruvate to acetate and CO2; CO to acetate and butyrate; vanillin to butyrate, protocatechuic aldehyde, and protocatechuate; ferulate to butyrate, caffeate, and hydrocaffeate; and syringate and 3,4,5-trimethoxybenzoate to butyrate and gallate. This new species did not use any other energy source, such as sugars, amino acids, other organic acids (including formate), methanol, ethanol, or H2-CO2. C. pfennigii is a small, motile, anaerobic, gram-positive, monotrichous rod-shaped organism with a lateral to subterminal flagellum, oval subterminal to terminal spores, and a deoxyribonucleic acid guanine-plus-cytosine content of 38 mol%. It did not liquefy gelatin. Based on the features described above, C. pfennigii may be closely related to Acetobacterium woodii. However, strain V5-2T (T = type strain) used pyruvate but did not use sugars or one-carbon compounds other than CO; it produced acetate and butyrate. The stoichiometry of substrate utilization and the growth yields from different energy sources are discussed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-35-4-454