RT Journal Article SR Electronic(1) A1 Mathrani, Indra M. A1 Boone, David R. A1 Mah, Robert A. A1 Fox, George E. A1 Lau, Paul P.YR 1988 T1 Methanohalophilus zhilinae sp. nov., an Alkaliphilic, Halophilic, Methylotrophic Methanogen JF International Journal of Systematic and Evolutionary Microbiology, VO 38 IS 2 SP 139 OP 142 DO https://doi.org/10.1099/00207713-38-2-139 PB Microbiology Society, SN 1466-5034, AB Methanohalophilus zhilinae, a new alkaliphilic, halophilic, methylotrophic species of methanogenic bacteria, is described. Strain WeN5T (T = type strain) from Bosa Lake of the Wadi el Natrun in Egypt was designated the type strain and was further characterized. This strain was nonmotile, able to catabolize dimethylsulfide, and able to grow in medium with a methyl group-containing substrate (such as methanol or trimethylamine) as the sole organic compound added. Sulfide (21 mM) inhibited cultures growing on trimethylamine. The antibiotic susceptibility pattern of strain WeN5T was typical of the pattern for archaeobacteria, and the guanine-plus-cytosine content of the deoxyribonucleic acid was 38 mol%. Characterization of the 16S ribosomal ribonucleic acid sequence indicated that strain WeN5T is phylogenetically distinct from members of previously described genera other than Methanohalophilus and supported the partition of halophilic methanogens into their own genus., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-38-2-139