Distribution and Phylogenetic Significance of 27-Hydroxy-Octacosanoic Acid in Lipopolysaccharides from Bacteria Belonging to the Alpha-2 Subgroup of Proteobacteria RAMADAS BHAT, U. and CARLSON, R. W. and BUSCH, M. and MAYER, H.,, 41, 213-217 (1991), doi = https://doi.org/10.1099/00207713-41-2-213, publicationName = Microbiology Society, issn = 1466-5026, abstract= Lipopolysaccharides (LPSs) from a number of bacteria belonging to the alpha-2 subgroup of the class Proteobacteria were screened for the presence of 27-hydroxy-octacosanoic acid (27-OH-28:0). With few exceptions, most of the bacteria contained 27-OH-28:0 in their lipid A fractions. In addition, some of the bacteria contained other n-2-hydroxylated long-chain fatty acids hitherto not reported. The distribution of 27-OH-28:0 was restricted to the alpha-2 subgroup. LPSs from members of the other subgroups (the beta and gamma subgroups), including some well-characterized enterobacterial LPSs, were devoid of 27-OH-28:0. Our results indicate that the presence of n-2-hydroxylated long-chain fatty acids in LPSs might be used as a chemophylogenetical marker., language=, type=