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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 41, Issue 3

Polyphasic Taxonomic Study of the Emended Genus : Relationship to , E. Falsen Group 10, and Other Clinical Isolates Free

Abstract

We used DNA-rRNA hybridization, DNA base composition, polyacrylamide gel electrophoresis of whole-cell proteins, DNA-DNA hybridization, numerical analysis of phenotypic features, and immunotyping to study the taxonomy of the genus . The relationships of this genus to and a group of clinical isolates (E. Falsen group 10 [EF 10]) were studied. Our DNA and rRNA hybridization results indicate that the genus consists of at least the following five genotypic groups: (i) , (ii) , (iii) , (iv) and a number of EF 10 strains, and (v) other EF 10 strains, several unnamed clinical isolates, and some misnamed strains of and subsp. . The existence of these five groups was confirmed by the results of immunotyping and protein gel electrophoresis. A numerical analysis of morphological, auxanographic, and biochemical data for the same organisms revealed the existence of three large phena. Two of these phena ( and ) correspond to two of the genotypic groups. The third phenon contains strains belonging to the other three genotypic groups, including most EF 10 strains and the type strains of and . The strains belonging to the third phenon were all incorporated into , and we propose that the use of the name should be discontinued. Emended descriptions of the genus and are presented.

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Copyright © 1991 International Union of Microbiological Societies
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1991-07-01
2024-04-20
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References

  1. Davis G. H. G., Park R. W. A. 1962; A taxonomic study of certain bacteria currently classified as Vibrio species. J. Gen. Microbiol. 27:101–119
     [Google Scholar]
  2. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
     [Google Scholar]
  3. De Ley J. 1978; Modern molecular methods in bacterial taxonomy: evaluation, application, prospects. 347–357Proceedings of the 4th International Conference of Plant Pathogenic Bacteria, Angers, vol. 1Gibert-Clarey, Tours, France
     [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
     [Google Scholar]
  5. De Ley J., De Smedt J. 1975; Improvements of the membrane filter method for DNA:rRNA hybridization. Antonie van Leeuwenhoek J. Microbiol. Serol. 41:287–307
     [Google Scholar]
  6. De Ley J., Van Muylem J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek J. Microbiol. Serol. 29:344–358
     [Google Scholar]
  7. De Vos P., De Ley J. 1983; Intra- and intergeneric similarities of Pseudomonas and Xanthomonas ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 33:487–509
     [Google Scholar]
  8. De Vos P., Goor M., Gillis M., De Ley J. 1985; Ribosomal ribonucleic acid cistron similarities of phytopathogenic Pseudomonas species. Int. J. Syst. Bacteriol. 35:169–184
     [Google Scholar]
  9. De Vos P., Kersters K., Falsen E., Pot B., Gillis M., Segers P., De Ley J. 1985; Comamonas Davis and Park 1962 gen. nov., nom. rev. emend., and Comamonas terrigena Hugh 1962 sp. nov., nom. rev. Int. J. Syst. Bacteriol. 35:443–453
     [Google Scholar]
  10. Falsen E. 1983 Immunodiffusion as an aid in routine identification of uncommon aerobic gram negative bacteria. 477–483 Leclerc H.ed Gram negative bacteria of medical and public health importance: taxonomy-identification-applications Les éditions de 1′Institut National de la Santé et de la Recherche Médicale; Paris:
     [Google Scholar]
  11. Falsen E. 1989 Catalogue of strains. Culture Collection, University of Göteborg; Göteborg, Sweden:
     [Google Scholar]
  12. Ferragut C., Kersters K., De Ley J. 1989; Protein electrophoretic and DNA homology analysis of Klebsiella strains. Syst. Appl. Microbiol. 11:121–127
     [Google Scholar]
  13. Gavini F., Holmes B., Izard D., Beji A., Bernigaud A., Jakubczak E. 1989; Numerical taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and related bacteria. Int. J. Syst. Bacteriol. 39:135–144
     [Google Scholar]
  14. Gilardi G. L. 1985 Pseudomonas,. 350–372 Lennette E. H., Balows A., Hausler W. J. Jr., Shadomy H. J.ed Manual of clinical microbiology American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  15. Goor M., Willems A., Gillis M. Unpublished data
  16. Hugh R. 1962; Comamonas terrigena comb. nov. with proposal of a neotype and request for an opinion. Int. Bull. Bacteriol. Nomenci. Taxon. 12:33–35
     [Google Scholar]
  17. Hylemon P. B., Wells J. S. Jr., Krieg N. R., Jannasch H. W. 1973; The genus Spirillum’, a taxonomic study. Int. J. Syst. Bacteriol. 23:340–380
     [Google Scholar]
  18. Kersters K., De Ley J. 1984 Genus Alcaligenes Castellani and Chalmers 1919. 361–373 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
     [Google Scholar]
  19. Kersters K., Hinz K.-H., Hertle A., Segers P., Lievens A., Siegmann O., De Ley J. 1984; Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. Int. J. Syst. Bacteriol. 34:56–70
     [Google Scholar]
  20. Kiredjian M., Holmes B., Kersters K., Guilvout I., De Ley J. 1986; Alcaligenes piechaudii, a new species from human clinical specimens and the environment. Int. J. Syst. Bacteriol. 36:282–287
     [Google Scholar]
  21. Krieg N. R. 1984 Aquaspirillum Hylemon, Wells, Krieg and Jannasch 1973. 72–90 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
     [Google Scholar]
  22. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
     [Google Scholar]
  23. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A.ed 1975 International code of nomenclature of bacteria. 1975. Revision. American Society for Microbiology; Washington, D.C:
     [Google Scholar]
  24. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
     [Google Scholar]
  25. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
     [Google Scholar]
  26. Owen R. J., Morgan D. D., Costas M., Lastovica A. 1989; Identification of “Campylobacter upsaliensis’ and other catalase-negative Campylobacters from pediatric blood cultures by numerical analysis of electrophoretic protein patterns. FEMS Microbiol. Lett. 58:145–150
     [Google Scholar]
  27. Oyaizu H., Komagata K. 1983; Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. J. Gen. Appl. Microbiol. 29:17–40
     [Google Scholar]
  28. Palleroni N. J. 1984 Genus I. Pseudomonas Migula 1894. 141–199 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
     [Google Scholar]
  29. Pickett M. J., Greenwood J. R. 1986; Pseudomonas alcaligenes and Pseudomonas testosterone, characterization and identification. Curr. Microbiol. 13:197–201
     [Google Scholar]
  30. Pot B., Gillis M., Hoste B., Van De Velde A., Bekaert F., Kersters K., De Ley J. 1989; Intra- and intergeneric relationships of the genus Oceanospirillum. Int. J. Syst. Bacteriol. 39:23–34
     [Google Scholar]
  31. Pot B., Willems A., Gillis M., De Ley J. Submitted for publication
  32. Ralston-Barrett E., Palleroni N. J., Doudoroff M. 1976; Phenotypic characterization and deoxyribonucleic acid homologies of the ““Pseudomonas alcaligenes” group. Int. J. Syst. Bacteriol. 26:421–426
     [Google Scholar]
  33. Rogers D. J., Tanimoto T. T. 1960; A computer program for classifying plants. Science 132:1115–1118
     [Google Scholar]
  34. Rossau R., Kersters K., Falsen E., Jantzen E., Segers P., Union A., Nehls L., De Ley J. 1987; Oligella, a new genus including Oligella urethralis comb. nov. (formerly Moraxella urethralis) and Oligella ureolytica sp. nov. (formerly CDC group IVe): relationship to Taylorella equigenitalis and related taxa. Int. J. Syst. Bacteriol. 37:198–210
     [Google Scholar]
  35. Rossau R., Vandenbussche G., Thielemans S., Segers P., Grosch H., Gothe E., Mannheim W., De Ley J. 1989; Ribosomal ribonucleic acid cistron similarities and deoxyribonucleic acid homologies of Neisseria, Kingella, Eikenella, Simonsiella, Alysiella, and Centers for Disease Control groups EF-4 and M-5 in the emended family Neisseriaceae. Int. J. Syst. Bacteriol. 39:185–198
     [Google Scholar]
  36. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy. The principles and practice of numerical classification W. H. Freeman and Co.; San Francisco:
     [Google Scholar]
  37. Stackebrandt E., Murray R. G. E., Triiper H. G. 1988; Proteobacteria classis nov., a name for the phylogenetic taxon that includes the “purple bacteria and their relatives.”. Int. J. Syst. Bacteriol. 38:321–325
     [Google Scholar]
  38. Tamaoka J., Ha D.-M., Komagata K. 1987; Reclassification of Pseudomonas acidovorans den Dooren de Jong 1926 and Pseudomonas testosteroni Marcus and Talalay 1956 as Comamonas acidovorans comb. nov. and Comamonas testosteroni comb, nov., with an emended description of the genus Comamonas. Int. J. Syst. Bacteriol. 37:52–59
     [Google Scholar]
  39. Vandamme P., Falsen E., Pot B., Hoste B., Kersters K., De Ley J. 1989; Identification of EF group 22 Campylobacters from gastroenteritis cases as Campylobacter concisus. J. Clin. Microbiol. 27:1775–1781
     [Google Scholar]
  40. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J. Gen. Microbiol. 129:3057–3074
     [Google Scholar]
  41. Willems A., Busse J., Goor M., Pot B., Falsen E., Jantzen E., Hoste B., Gillis M., Kersters K., Auling G., De Ley J. 1989; Hydrogenophaga, a new genus of hydrogen-oxidizing bacteria that includes Hydrogenophaga flava comb. nov. (formerly Pseudomonas flava), Hydrogenophaga palleronii (formerly Pseudomonas palleronii), Hydrogenophaga pseudoflava (formerly Pseudomonas pseudoflava and “Pseudomonas carboxydoflava”), and Hydrogenophaga taeniospiralis (formerly Pseudomonas taeniospiralis). Int. J. Syst. Bacteriol. 39:319–333
     [Google Scholar]
  42. Willems A., De Vos P., De Ley J., Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K. H.ed The prokaryotes, 2nd. in press
    [Google Scholar]
  43. Willems A., Falsen E., Pot B., Jantzen E., Hoste B., Vandamme P., Gillis M., Kersters K., De Ley J. 1990; Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, EF group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb, nov., Acidovorax delafieldii comb, nov., and Acidovorax temperans sp. nov.. Int. J. Syst. Bacteriol. 40:384–398
     [Google Scholar]
  44. Willems A., Gillis M., De Ley J. 1991; Transfer of Rhodocyclus gelatinosas to Rubrivivax gelatinosas gen. nov., comb, nov., and phylogenetic relationships with Leptothrix, Sphaerotilus natans, Pseudomonas saccharophila, and Alcaligenes latus. Int. J. Syst. Bacteriol. 41:65–73
     [Google Scholar]
  45. Willems A., Gillis M., Kersters K., Van den Broecke L., De Ley J. 1987; Transfer of Xanthomonas ampelina Panagopoulos 1969 to a new genus, Xylophilus gen. nov., as Xylophilus ampelinus (Panagopoulos 1969) comb. nov.. Int. J. Syst. Bacteriol. 37:422–430
     [Google Scholar]
  46. Wishart D. 1978 Clustan user manual. , 3rd. Program Library Unit; Edinburgh University, Edinburgh, Scotland:
     [Google Scholar]
  47. Woese C. R., Weisburg W. G., Paster B. J., Hahn C. M., Tanner R. S., Krieg N. R., Koops H.-P., Harms H., Stackebrandt E. 1984; The phylogeny of purple bacteria: the beta subdivision. Syst. Appl. Microbiol. 5:327–336
     [Google Scholar]
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Polyphasic Taxonomic Study of the Emended Genus Comamonas: Relationship to Aquaspirillum aquaticum, E. Falsen Group 10, and Other Clinical Isolates
Int J Syst Evol Microbiol 41, 427 (1991); https://doi.org/10.1099/00207713-41-3-427
/content/journal/ijsem/10.1099/00207713-41-3-427
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