1887

Abstract

The partial 16S rRNA sequence of an unknown bacterium that was originally isolated from middle ear fluids of children with persistent otitis media was determined by reverse transcription. A comparison of this sequence with sequences from other gram-positive species having low guanine-plus-cytosine contents revealed that this bacterium represents a new line of descent, for which the name gen. nov., sp. nov., is proposed. The type strain is strain NCFB 2890.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-42-1-79
1992-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/42/1/ijs-42-1-79.html?itemId=/content/journal/ijsem/10.1099/00207713-42-1-79&mimeType=html&fmt=ahah

References

  1. Bentley R. W., Leigh J. A., Collins M. D. 1991; Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int. J. Syst. Bacteriol. 41:489–494
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75:4801–4805
    [Google Scholar]
  3. Collins M. D., Ash C., Farrow J. A. E., Wallbanks S., Williams A. M. 1990; 16S ribosomal ribonucleic acid sequence analyses of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov. J. Appl. Bacteriol. 67:453–460
    [Google Scholar]
  4. Collins M. D., Wallbanks S., Lane D. J., Shah J., Nietupski R., Smida J., Dorsch M., Stackebrandt E. 1991; Phylogenetic analysis of the genus Listeria based on reverse transcriptase sequencing of 16S rRNA. Int. J. Syst. Bacteriol. 41:240–246
    [Google Scholar]
  5. Collins M. D., Williams A. M., Wallbanks S. 1990; The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen.nov. FEMS Microbiol. Lett. 70:255–262
    [Google Scholar]
  6. Embley T. M., Smida J., Stackebrandt E. 1988; Reverse transcriptase sequencing of 16S ribosomal RNA from Faenia rectivirgula, Pseudonocardia thermophila and Saccharopolyspora hirsuta, three wall IV actinomycètes which lack mycolic acids. J. Gen. Microbiol. 134:961–966
    [Google Scholar]
  7. Faden H., Dryja D. 1989; Recovery of a unique bacterial organism in human middle ear fluid and its possible role in chronic otitis media. J. Clin. Microbiol. 27:2488–2491
    [Google Scholar]
  8. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
    [Google Scholar]
  9. Garvie E. I. 1976; Hybridization between the deoxyribonucleic acids of some strains of heterofermentative lactic acid bacteria. Int. J. Syst. Bacteriol. 26:116–122
    [Google Scholar]
  10. Martinez-Murcia A. J., Collins M. D. 1990; A phylogenetic analysis of the genus Leuconostoc based on reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol. Lett. 70:73–84
    [Google Scholar]
  11. Queen C., Korn L. J. 1984; A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Res. 12:581–599
    [Google Scholar]
  12. Wallbanks S., Martinez-Murcia A. J., Fryer J. L., Phillips B. A., Collins M. D. 1990; 16S ribosomal ribonucleic acid sequence determination of members of the genus Carnobacterium and related lactic acid bacteria. Description of Vagococcus salmoninarum sp. nov. Int. J. Syst. Bacteriol. 40:224–230
    [Google Scholar]
  13. Whiley R. A., Fraser H. Y., Douglas C. W. I., Hardie J. M., Williams A. M., Collins M. D. 1990; Streptococcus parasanguis sp. nov., an atypical viridans Streptococcus from human clinical specimens. FEMS Microbiol. Lett. 68:115–122
    [Google Scholar]
  14. Williams A. M., Collins M. D. 1990; Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov. J. Appl. Bacteriol. 68:485–490
    [Google Scholar]
  15. Williams A. M., Farrow J. A. E., Collins M. D. 1989; Reverse transcriptase sequencing of 16S ribosomal RNA from Streptococcus cecorum. Lett. Appl. Microbiol. 8:185–189
    [Google Scholar]
  16. Williams A. M., Rodrigues U. M., Collins M. D. 1991; Intrageneric relationships of enterococci as determined by reverse transcriptase sequencing of small-subunit rRNA. Res. Microbiol. 142:67–74
    [Google Scholar]
  17. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev. 51:221–271
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-42-1-79
Loading
/content/journal/ijsem/10.1099/00207713-42-1-79
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error