1887

Abstract

Abstract

The levels of DNA relatedness for a broad sample of strains isolated from different species of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) and from different geographical sources were estimated by the hydroxyapatite method. The level of DNA-DNA relatedness for the two phases of each isolate tested was not significantly different from 100%, demonstrating unequivocally that the phase variation demonstrated by all spp. is not due to contamination. The isolates of the described species coalesced into different DNA relatedness groups, confirming that , , and , defined on the basis of phenotypic differences, are valid species. The symbiont of also coalesced with the isolates. This was the only symbiont of seven recently described and unamed spp. (including , and ) that formed a group with any of the previously described species; new species descriptions are required to accommodate the other taxa, but too few isolates were available to allow satisfactory descriptions of them. The DNA relatedness data also showed that the bacteria currently classified as are significantly different from all other strains. These data strongly support indications from previous studies of phenotypic characteristics, cellular fatty acids, and DNA relatedness that should be classified as a separate genus. A new genus, , with an amended description of the type species, , is proposed.

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1993-04-01
2024-03-29
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