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Volume 43, Issue 4

sp. nov. Is Related to and Found in Birds Free

Abstract

A mycoplasma designated strain 4229(T = type strain) was isolated in 1984 from the turbinate of a duck in France, and similar strains were isolated from geese in France and from a partridge in England. All of these strains were originally identified as by immunofluorescence and growth inhibition tests, but subsequent serological and molecular studies indicated only a partial relationship to this species and DNA-DNA hybridization studies revealed only approximately 40 to 46% genetic homology with PG31. In this study morphological, cultural, and physical investigations were carried out on strain 4229and partridge strain B2/85, after we first demonstrated the similarity between these organisms by performing a restriction enzyme analysis of their DNAs. Both strains had phenotypic properties very similar to properties, including the presence of an attachment organelle. As a result of these investigations, the organisms were assigned to the class , the order , and the genus They fermented glucose, reduced triphenyl tetrazolium chloride aerobically and anaerobically, and exhibited hemadsorption and hemagglutination, but other biochemical tests were negative. Apart from a serological cross-reaction with , these organisms exhibited no significant relationship with any previously described species as determined by growth inhibition or immunofluorescence tests or with a number of additional serovars and unclassified avian strains. This taxon therefore appears to be a new species, for which we propose the name The type strain is strain 4229 (= NCTC 11733 = ATCC 51306). The significance of the organism has not been fully investigated, but preliminary in vitro and in vivo studies have indicated that it may be pathogenic.

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References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
     [Google Scholar]
  2. Askaa G., Erno H. 1976; Elevation of Mycoplasma agalactiae subsp bovis to species rank: Mycoplasma bovis (Hale et al.) comb. nov. Int. J. Syst. Bacteriol. 26:322–325
     [Google Scholar]
  3. Bentina D., Dorrer D., Tadina T. 1987; Mycoplasma species isolated from six avian species. Avian Pathol. 16:653–664
     [Google Scholar]
  4. Bencina D., Tadina T., Dorrer D. 1988; Natural infection of ducks with Mycoplasma synoviae and Mycoplasma gallisepticum and Mycoplasma egg transmission. Avian Pathol. 17:441–449
     [Google Scholar]
  5. Bencina D., Tadina T., Dorrer D. 1988; Natural infection of geese with Mycoplasma gallisepticum and Mycoplasma synoviae and egg transmission of the mycoplasmas. Avian Pathol. 17:925–928
     [Google Scholar]
  6. Black F. T. 1973; Modification of the growth inhibition test and its application to human T-mycoplasmas. Appl. Microbiol. 25:528–533
     [Google Scholar]
  7. Bradbury J. M. 1977; Rapid biochemical tests for characterization of the Mycoplasmatales. J. Clin. Microbiol. 5:531–534
     [Google Scholar]
  8. Bradbuiy J. M. 1984; Avian mycoplasma infections: prototype of mixed infections with mycoplasmas, bacteria and viruses. Ann. Microbiol. (Inst. Pasteur) 135A:83–89
     [Google Scholar]
  9. Bradbury J. M. Unpublished data
  10. Bradbury J. M., Forrest M., Williams A. 1983; Mycoplasma lipofaciens, a new species of avian origin. Int. J. Syst. Bacteriol. 33:329–335
     [Google Scholar]
  11. Buntz B. 1987; Isolement et mise en evidence du pouvoir pathogene de M. gallisepticum chez l’oie (Anser anser). Ph.D. thesis. Universite de Bordeaux II, Villenave d’Ornon, France
    [Google Scholar]
  12. Buntz B., Bradbury J. M., Vuillaume A., Rousselot-Paillet D. 1986; Isolation of Mycoplasma gallisepticum from geese. Avian Pathol. 15:615–617
     [Google Scholar]
  13. Coie R. M. 1983; Transmission electron microscopy: basic techniques. Methods Mycoplasmol. 1:43–50
     [Google Scholar]
  14. Costas M., Leach R. H., Mitchelmore D. L. 1987; Numerical analysis of PAGE protein patterns and the taxonomic relationships within the “Mycoplasma mycoides cluster.”. J. Gen. Microbiol. 133:3319–3329
     [Google Scholar]
  15. Dove P., Bencina D., Antes R., Mann W. 1992; Recombinant DNA probes and PCR for detection of Mycoplasma gallisepticum strains. Int. Org. Mycoplasmol. Lett. 2:109
     [Google Scholar]
  16. Dupiellet J.-P. 1984; Mycoplasmes et acholeplasmes des palmipedes a foie gras: isolement, caracterisation, etude du role dans la pathologie. Rapport de D.E.A. de Pathologie. Universite de Bordeaux II, Villenave d’Ornon; France:
     [Google Scholar]
  17. Dupiellet J.-P. 1988; Mycoplasmes de l’oie et du canard: contribution a l’etude serologique et moleculaire de souches aparentees a Mycoplasma gallisepticum. Ph.D. thesis. Universite de Bordeaux II, Villenave d’Ornon, France
    [Google Scholar]
  18. Dupiellet J.-P., Vuillaume A., Rousselot D., Bove J. M., Bradbury J. M. 1988; Serological and molecular studies on Mycoplasma gallisepticum strains, p. 150. Abstr. 7th Congr. Int. Org. Mycoplasmol. International Organization for Myco-plasmology
    [Google Scholar]
  19. Dupiellet J. P., Vuillaume A., Rousselot D., Bove J. M., Bradbuiy J. M. 1990; Serological and molecular studies on Mycoplasma gallisepticum strains. Zentralbl. Bakteriol. Suppl. 20:859–864
     [Google Scholar]
  20. Edward D. G. ff., Moore W. B. 1975; A method for determining utilization of glucose by mycoplasmas. J. Med. Microbiol. 8:451–454
     [Google Scholar]
  21. Erno H., Stipkovits L. 1973; Bovine mycoplasmas. Cultural and biochemical studies. I. Acta Vet. Scand. 19:436–449
     [Google Scholar]
  22. Fernandez C., Mattsson J. G., Bolske G., Levisohn S., Johansson K.-E. Species-specific oligonucleotide probes complementary to 16S rRNA of Mycoplasma gallisepticum and Mycoplasma synoviae. Res. Vet. Sci. in press
    [Google Scholar]
  23. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium polyanetholsulfonate and digitonin. Zentralbl. Bakteriol. Para-sitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 255:104–112
     [Google Scholar]
  24. Freundt E. A., Erno H., Lemcke R. M. 1979; Identification of mycoplasmas. Methods Microbiol. 13:377–434
     [Google Scholar]
  25. Gardella R. S., DelGiudice R. A. 1983; Hemagglutination, hemadsorption and hemolysis. Methods Mycoplasmol. 1:379–384
     [Google Scholar]
  26. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of MoUicutes 1979; Proposal of minimal standards for descriptions of new species of the class Molli-cutes. Int. J. Syst. Bacteriol. 29:172–180
     [Google Scholar]
  27. Johnson J. L. 1984; Bacterial classification. III. Nucleic acids in bacterial classification. p. 8–11 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 Williams and Wilkins; Baltimore:
     [Google Scholar]
  28. Jordan F. T. W., Amin M. M. 1980; A survey of mycoplasma infections in domestic poultry. Res. Vet. Sci. 28:96–100
     [Google Scholar]
  29. Kleven S. H., Browning G. F., Bulach D. M., Ghiocas E., Morrow C. J., Whithear K. G. 1988; Examination of Mycoplasma gallisepticum strains using restriction endonuclease DNA analysis and DNA-DNA hybridization. Avian Pathol. 17:559–570
     [Google Scholar]
  30. Kleven S. H., Morrow C. J., Whithear K. G. 1988; Comparison of Mycoplasma gallisepticum strains by hemagglu- tination-inhibition and restriction enzyme analysis. Avian Dis. 32:731–741
     [Google Scholar]
  31. Livingston C. W. 1972; Isolation of T-strain mycoplasma from Texas feedlot cattle. Am. J. Vet. Res. 33:1925–1929
     [Google Scholar]
  32. Madden D. L., Henderson W. H., Moses H. E. 1967; Case report: isolation of Mycoplasma gallisepticum from bobwhite quail (Colinus virginianus). Avian Dis. 11:378–380
     [Google Scholar]
  33. Osborn O. H., Pomeroy B. S. 1958; Isolation of the agent of infectious sinusitis of turkeys from naturally infected pheasants. Avian Dis. 2:370–372
     [Google Scholar]
  34. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol. 102:306–310
     [Google Scholar]
  35. Reece R. L., Ireland L., Barr D. A. 1986; Infectious sinusitis associated with Mycoplasma gallisepticum in gamebirds. Aust. Vet. J. 63:167–168
     [Google Scholar]
  36. Rosendal S., Black F. T. 1972; Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta Pathol. Microbiol. Scand. Sect. B 80:615–622
     [Google Scholar]
  37. Saed O. M., Bradbury J. M. Unpublished data
  38. Saed O. M., Ross G., Bradbury J. M. 1990; Pathogenicity studies on an avian mycoplasma related to Mycoplasma gallisepticum. Int. Org. Mycoplasmol. Lett. 1:547–548
     [Google Scholar]
  39. Shepard M. C., Howard D. R. 1970; Identification of “T” mycoplasmas in primary agar cultures by means of a direct test for urease. Ann. N.Y. Acad. Sci. 174:809–819
     [Google Scholar]
  40. Tiong S. K. 1978; Isolation of Mycoplasma gallisepticum from sinuses of three quail (Cortumix cortunix japonica). Vet. Rec. 103:539
     [Google Scholar]
  41. Tully J. G., Whitcomb R. G., Clark H. F., Williamson D. L. 1977; Pathogenic mycoplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. Science 195:892–894
     [Google Scholar]
  42. Van Roekel H., Olesiuk O. M. 1953; The etiology of chronic respiratory disease. p. 289–303 In Proceedings of the 90th Annual Meeting of the American Veterinary Medicine Association American Veterinary Medical Association; Chicago:
     [Google Scholar]
  43. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., £. Murray R. G., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
     [Google Scholar]
  44. Wichmann R. W. 1957; Case report—PPLO infection in chukar partridges (Alectoris graeca). Avian Dis. 1:222–226
     [Google Scholar]
  45. Yagihashi T., Nunoya T., Tajima M. 1988; Pathogenicity for chickens of six strains of Mycoplasma gallisepticum isolated from various birds. Avian Pathol. 17:725–729
     [Google Scholar]
  46. Yoder H. W. Jr., Hofstad M. S. 1964; Characterisation of avian mycoplasmas. Avian Dis. 8:481–512
     [Google Scholar]
  47. Yogev D., Levisohn S., Kleven S. H., Halachmi D., Razin S. 1988; Ribosomal RNA gene probes to detect intraspecies heterogeneity in Mycoplasma gallisepticum and M. synoviae. Avian Dis. 32:220–231
     [Google Scholar]
  48. Yogev D., Sela S., Bercovier H., Razin S. 1988; Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains. FEMS Microbiol. Lett. 50:145–149
     [Google Scholar]
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Mycoplasma imitans sp. nov. Is Related to Mycoplasma gallisepticum and Found in Birds
Int J Syst Evol Microbiol 43, 721 (1993); https://doi.org/10.1099/00207713-43-4-721
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