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Volume 45, Issue 3

Phylogenetic Analyses of a New Group of Denitrifiers Capable of Anaerobic Growth on Toluene and Description of sp. nov. Free

Abstract

To understand the phylogeny and taxonomy of eight new toluene-degrading denitrifying isolates, we performed a 16S rRNA sequence analysis and a gas chromatographic analysis of their cellular fatty acids and examined some of their biochemical and physiological features. These isolates had 16S rRNA sequence signatures identical to those of members of the beta subclass of the The levels of similarity were as follows: 97.9 to 99.9% among the new isolates; 91.2 to 92.4% between the new isolates and sp. strain S5b2; 95.3 to 96.2% between the new isolates and sp. strain BH72; and 94.8 to 95.3% between the new isolates and VB32 (T = type strain). Phylogenetic trees constructed by using the distance matrix, maximum-parsimony, and maximum-likelihood methods showed that our eight denitrifying isolates form a phylogenetically coherent cluster which represents a sister lineage of the previously described species. Furthermore, the fatty acid profiles, the cell morphology, and several physiological and nutritional characteristics of the eight isolates and the previously described members of the genus were also similar. In contrast to the previously described members of the genus the eight new isolates were capable of degrading toluene under denitrifying conditions. We concluded that these toluene-degrading denitrifiers are members of a new species of the novel nitrogen-fixing genus We propose the name for these toluene-degrading denitrifying isolates and designate strain Tol-4 the type strain.

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Copyright © 1995 International Union of Microbiological Societies
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1995-07-01
2024-04-18
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Phylogenetic Analyses of a New Group of Denitrifiers Capable of Anaerobic Growth on Toluene and Description of Azoarcus tolulyticus sp. nov.
Int J Syst Evol Microbiol 45, 500 (1995); https://doi.org/10.1099/00207713-45-3-500
/content/journal/ijsem/10.1099/00207713-45-3-500
/content/journal/ijsem/10.1099/00207713-45-3-500
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