1887

Abstract

Almost complete (>96%) 16S rRNA sequences from nine ruminant mycoplasmas have been determined by solid-phase DNA sequencing. Polymorphisms were found in four of the 16S rRNA sequences, which indicated the existence of two different rRNA operons. Seven polymorphisms were found in , three were found in , one was found in , and one was found in . The sequence data were used for construction of phylogenetic trees. All but one of the ruminant mycoplasmas sequenced in this work clustered in the hominis group. A close relationship was found between and , with a 99% nucleotide similarity between their 16S rRNA sequences. They were also found to be members of the cluster of the hominis group. Furthermore, the 16S rRNA comparisons showed that and are closely related (>98.5%), and these species were found to cluster in the cluster of the hominis group. Interestingly, grouped in a new phylogenetic cluster of the hominis group. The new cluster, which was supported by bootstrap percentage values, signature nucleotide analysis, and higher-order structural elements, was named the cluster. , and clustered in the cluster of the hominis group. clustered with in the cluster of the pneumoniae group.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-4-1093
1996-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/4/ijs-46-4-1093.html?itemId=/content/journal/ijsem/10.1099/00207713-46-4-1093&mimeType=html&fmt=ahah

References

  1. Askaa G., Erno H. 1976; Elevation of Mycoplasma agalactiae subsp. bovis to species rank: Mycoplasma bovis (Hale et al.) comb. nov. Int. J. Syst. Bacteriol 26:323–325
    [Google Scholar]
  2. Bolske G. 1988; Survey of mycoplasma infections in cell cultures and a comparison of detection methods. Zentralbl. Bakteriol. Hyg. A 269:331–340
    [Google Scholar]
  3. Boughton E. 1979; Mycoplasma bovis mastitis. Vet. Bull 49:377–387
    [Google Scholar]
  4. Brosius J., Palmer J. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75:4801–4805
    [Google Scholar]
  5. Felsenstein J. 1993 PHYLIP: phylogeny inference package (version 3.51c) Department of Genetics; University of Washington, Seattle:
    [Google Scholar]
  6. Gutell R. R. 1994; Collection of small (16S- and 16S-like) ribosomal RNA structures: 1994. Nucleic Acids Res 22:3502–3507
    [Google Scholar]
  7. Hultman T., Bergh S., Moks T., Uhlen M. 1991; Bidirectional solid phase sequencing of in vziro-amplified plasmid DNA. BioTechniques 10:8493
    [Google Scholar]
  8. Hultman T., Stahl S., Hornes E., Uhlen M. 1989; Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res 17:4937–4946
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism 3 Academic Press, Inc.; New York:
    [Google Scholar]
  10. Lambert M. 1987; Contagious agalactia of sheep and goats. Rev. Sci. Technol. Off. Int. Epizoot 6:677–711
    [Google Scholar]
  11. Maidak B. L., Larsen N., McCaughey M. J., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res 22:3485–3487
    [Google Scholar]
  12. Maniloff J. 1992 Phylogeny of mycoplasmas. 549–559 Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B.ed Mycoplasmas: molecular biology and pathogenesis American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  13. Mattsson J. G., Guss B., Johansson K.-E. 1994; The phylogeny of Mycoplasma bovis as determined by sequence analysis of the 16S rRNA gene. FEMS Microbiol. Lett 115:325–328
    [Google Scholar]
  14. Olsen G. J., Woese C. R. 1993; Ribosomal RNA: a key to phylogeny. FASEB J 7:113–123
    [Google Scholar]
  15. Pettersson B., Johansson K.-E., Uhlen M. 1994; Sequence analysis of 16S rRNA from mycoplasmas by direct solid-phase DNA sequencing. Appl. Environ. Microbiol 60:2456–2461
    [Google Scholar]
  16. Pettersson B., Leitner T., Ronaghi M., Bolske G., Uhlen M., Johansson K.-E. 1996; Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons. J. Bacteriol 178:4131–4142
    [Google Scholar]
  17. Razin S. 1992 Mycoplasma taxonomy and ecology. 3–22 Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B.ed Mycoplasmas: molecular biology and pathogenesis American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  18. Ross R. F. 1993 Mycoplasma–animal pathogens. 69–109 Kahane I., Adoni A.ed Rapid diagnosis of mycoplasmas Plenum Press; New York:
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  20. Simecka J. W., Davis J. K., Davidsson M. K., Ross S. E., Stadtlander C. T. K.-H., Cassell G. H. 1992 Mycoplasma diseases of animals. 391–415 Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B.ed Mycoplasmas: molecular biology and pathogenesis American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  21. Ter Laak E., Wentink G. H., Zimmer G. M. 1992; Increased prevalence of Mycoplasma bovis in The Netherlands. Vet. Q 14:100–104
    [Google Scholar]
  22. Tully J. G., Bove J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutesi proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasmatales ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae fam. nov.) from helical species (Spiroplasmataceae), and emended descriptions of the order Mycoplasmatales, family Mycoplasmataceae. Int. J. Syst. Bacteriol 43:378–385
    [Google Scholar]
  23. Wahlberg J., Holmberg A., Bergh S., Hultman T., Uhlen M. 1992; Automated magnetic preparation of DNA templates for solid phase sequencing. Electrophoresis 13:547–551
    [Google Scholar]
  24. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol 171:6455–6467
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-4-1093
Loading
/content/journal/ijsem/10.1099/00207713-46-4-1093
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error